48-630MAG MILLIPLEXMAP NF-κB Signaling Magnetic Bead Kit 6-plex Kit - Cell Signaling Multiplex Assay

48-630MAG
96-well plate  96-well plate
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      Overview

      Replacement Information

      Key Spec Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      c-Myc, FADD (Ser194), IκBα (Ser32), IKKα/β (Ser177/Ser181), NFκB (Ser536), TNFR1 H Mplex Luminex xMAP
      Description
      Catalogue Number48-630MAG
      Trade Name
      • MILLIPLEX
      DescriptionMILLIPLEXMAP NF-κB Signaling Magnetic Bead Kit 6-plex Kit - Cell Signaling Multiplex Assay
      OverviewNF-κB (nuclear factor kappa B) is a transcription factor that plays a regulatory role in several physiological and pathological processes. The NF-κB pathway can be activated by numerous stimuli, including inflammatory cytokines, virus, bacteria, UV radiation, genotoxic stress, DNA damage, oxidative stress, etc. The IkBα/NF-κB complex is tightly regulated by both phosphorylation and ubiquitination. Phosphorylation and ubiquitination of IκBα leads to its degradation and subsequent release of phosphorylated NF-κB to the nucleus. Greater than 100 target genes, including cytokines, cyclins, p21, Bcl-2, and c-Myc are known to be transcribed by NF-kB.
      References
      Product Information
      Detection methodLuminex xMAP
      ConfigurationPremixed
      Panel TypeCell Signaling
      Quality LevelMQ200
      Applications
      ApplicationIntracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples. Compare Multiplexing results to those of Western blotting.
      Key Applications
      • Multiplexing
      Application Noteso This is an overnight (4°C) incubation assay.
      o This kit must be run using the provided Assay Buffer.
      o This assay requires 25 μL diluted filtered cell lysate per well.
      o The suggested working range of protein concentration for the assay is 1 to 25 µg of total protein/well (25 µL/well at 40 to 1,000 µg/mL).
      Biological Information
      SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
      Species Reactivity
      • Human
      Analytes Available
      • c-Myc
      • FADD (Ser194)
      • IκBα (Ser32)
      • IKKα/β (Ser177/Ser181)
      • NFκB (Ser536)
      • TNFR1
      Assay PanelPremixed beads for your convenience.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage for kit components is 2 - 8°C.
      Packaging Information
      Material Size96-well plate
      Material Package96-well plate
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Protocols

      Title
      NF-κB 6-PlexSignaling Magnetic Bead Kit - 96-well Plate

      MILLIPLEXMAP NF-κB Signaling Magnetic Bead Kit 6-plex Kit - Cell Signaling Multiplex Assay MSDS

      Title

      Safety Data Sheet (SDS) 

      Brochure

      Title
      A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with Merck Millipore Immunoassay Platform Solutions.
      Custom Immunoassay Menu of Services
      Pathways and Biomarkers of Toll-like Receptor (TLR) Signaling
      The Power of Biomarker Analysis

      Technical Info

      Title
      96 well Template 2 Up
      96 well Template Single

      Data Sheet

      Title
      Full Service Custom-built Assays

      Posters

      Title
      MILLIPLEX MAP: Exercise, Signaling, and Your Brain
      Analysis of NF-kB Signaling Using Luminex® xMAP® Technology
      MILLIPLEX® MAP Cell Signaling Wall Chart (MM)