Skip to Content
Merck

676604

PhosphoDetect Anti-VASP (pSer¹⁵⁷) Mouse mAb (5C6)

lyophilized, clone 5C6, Calbiochem®

Synonym(s):

Anti-Vasodilator-stimulated Phosphoprotein, Activated

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

NACRES:
NA.41
UNSPSC Code:
12352203

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

PhosphoDetect Anti-VASP (pSer¹⁵⁷) Mouse mAb (5C6), lyophilized, clone 5C6, Calbiochem®

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

5C6, monoclonal

form

lyophilized

contains

≤0.1% sodium azide as preservative

species reactivity

human, mouse

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

dilution

(ELISA (0.05 µg/mL)
Immunoblotting (1 µg/mL, colorimetric; 0.1 µg/mL, chemiluminescence)
Immunocytochemistry (1-10 µg/mL)
Immunoprecipitation (1-10 µg/10 vanadate-treated A431 cells))

isotype

IgG1

shipped in

ambient

storage temp.

−20°C

target post-translational modification

phosphorylation (pSer157)

Quality Level

100

Gene Information

human ... VASP(7408)

General description

Mouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography. Recognizes the the ~50 kDa VASP protein phosphorylated at Ser157.
Recognizes the the ~50 kDa VASP protein phosphorylated at Ser157.
This PhosphoDetect Anti-VASP (pSer¹⁵⁷) Mouse mAb (5C6) is validated for use in ELISA, Immunoblotting, ICC, IP for the detection of VASP (pSer¹⁵⁷).

Analysis Note

Positive Control
Vanadate-treated A431 cells

Application

ELISA (0.05 µg/ml)

Immunoblotting (1 µg/ml, colorimetric; 0.1 µg/ml, chemiluminescence)

Immunocytochemistry (1-10 µg/ml)

Immunoprecipitation (1-10 µg/10⁶ vanadate-treated A431 cells)

Disclaimer

Toxicity: Standard Handling (A)

Immunogen

Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹⁵⁷ phosphorylation site of human VASP

Other Notes

Bear, J.E., et al. 2001. Curr. Opin. Cell. Biol.13, 158.
Lawrence, D.W., and Pryzwansky, K.B. 2001. J. Immunol.166, 5550.
Burkhardt, M., et al. 2000. J. Biol. Chem.275, 33536.
Harbeck, B., et al. 2000. J. Biol. Chem.275, 40.
Smolenski, A., et al. 2000. J. Biol. Chem.275, 25723.
Does not cross-react with non-phosphorylated VASP or with unrelated phosphorylation sites. For immunocytochemistry, fixation protocol may include up to 0.5% formaldehyde. Variables associated with assay conditions will dictate the proper working dilution.

Physical form

Lyophilized from PBS, PEG, sucrose.

Preparation Note

Reconstitute in 1 ml sterile, dH₂O, 15 min, room temperature. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Not finding the right product?  

Try our Product Selector Tool.

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Min Li et al.
The Journal of biological chemistry, 290(50), 30030-30041 (2015-10-27)
Diabetes is strongly associated with cognitive decline, but the molecular reasons are unknown. We found that fasting and peripheral insulin promote phosphorylation and dephosphorylation, respectively, of specific residues on brain proteins including cytoskeletal regulators such as slit-robo GTPase-activating protein 3

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service