Skip to Content
Merck

Key Documents

View All Documentation

03-0177-00

SMC® Human IL-17A/F Heterodimer High Sensitivity Immunoassay Kit

Used for ultrasensitive quantification of Human IL-17A/F Heterodimer in human serum and plasma

Synonym(s):

IL-17A/F Single Molecule Counting Kit, IL-17A/F ultra-sensitive immunoassay

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

NACRES:
NA.84
UNSPSC Code:
12161503
Species reactivity:
human
Technique(s):
single molecule counting: suitable
Usage:
 kit sufficient for 96 wells
Compatibility:
for use with TM="FemtoQuest"] and SMCxPRO® instruments
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

SMC® Human IL-17A/F Heterodimer High Sensitivity Immunoassay Kit,

description

Used for ultrasensitive quantification of Human IL-17A/F Heterodimer in human serum and plasma

product line

SMC®

usage

 kit sufficient for 96 wells

species reactivity

human

technique(s)

single molecule counting: suitable

input

serum
plasma (K2 EDTA)

UniProt accession no.

Q16552

compatibility

for use with TM="FemtoQuest"] and SMCxPRO® instruments

shipped in

wet ice

storage temp.

2-8°C

General description

Interleukin-17A/F (IL-17A/F) is a heterodimeric cytokine formed by the combination of IL-17A and IL-17F subunits, both of which are produced primarily by Th17 cells. This cytokine plays a significant role in mediating inflammatory responses and is involved in the recruitment of neutrophils and other immune cells to sites of inflammation. IL-17A/F is implicated in various autoimmune diseases and chronic inflammatory conditions, highlighting its potential as a therapeutic target for managing such disorders.

Application

Inflammatory Disease / Immune Response / Autoimmune Disease

Features and Benefits

  • Specific Detection of IL-17A/F Heterodimer: Enables accurate quantification of the biologically distinct IL-17A/F complex, which plays a unique role in Th17-mediated inflammation and autoimmune disease.
  • Ultra-Sensitive Quantification via SMC®Technology: Supports early detection of immune activation, especially in diseases where IL-17A/F is present at low levels, such as psoriasis, multiple sclerosis, and IBD.
  • Verified for Human Serum and Plasma: Demonstrates high recovery rates (88–101%) in spiked human samples.

Packaging

96 well assay

Analysis Note

  • Sensitivity: LLOQ 2,340 fg/mL* (SMCxPRO® Instrument)
  • Standard Curve Range: 590-600,000 fg/mL
  • Intra-assay CV: <15%
  • Inter-assay CV: <20%
  • Linearity: Serum: 106% Plasma: 111%
  • Recovery: Serum: 101% Plasma: 88%
*Indicative LLOQ created from average of 14 independent in house runs, where >80% met same value defined with CV & Recovery +/- 20%. Refer to CoA for lot specific performance.

Other Notes

Although IL-17A/F Heterodimer was detectable in non-human primates, monkey and canine samples, the assay has not been verified for these species.

Legal Information

SMC is a registered trademark of Singulex
SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

signalword

Danger

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2 - Repr. 1B

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Global Trade Item Number

SKUGTIN
03-0177-0004054839759352