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662103 UbcH13 Conjugating Enzyme E2, His•Tag®, Human, Recombinant, E. coli

UbcH13 Conjugating Enzyme E2, His•Tag®, Human, Recombinant, E. coli MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Ubc13, UBE2N, Ubiquitin-conjugating enzyme E2 N

662103
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      Full-length, recombinant, human UbcH13 fused at the N-terminus to a His•Tag® sequence and expressed in E. coli. UbcH13, an E2 ubiquitin ligase, forms a heterodimeric complex with Uev1, and is able to assemble K63-linked polyubiquitin chains, which have novel regulatory roles. These ubiquitin chains have a role in a variety of processes such as DNA repair, endocytosis, polysome stability, and signal transduction pathways. The active enzyme is useful in the study of ubiquitin regulation and inhibitor screening.
      Catalogue Number662103
      Brand Family Calbiochem®
      SynonymsUbc13, UBE2N, Ubiquitin-conjugating enzyme E2 N
      References
      ReferencesMcKenna, S., et al. 2003. Biochem. 42, 7922.
      Hofmann, R.M., and Pickart C.M. 2001. J. Biol. Chem. 276, 27936.
      McKenna, S., et al. 2001. J. Biol. Chem. 276, 40120.
      Moraes, T.F., et al. 2001. Nat. Struc. Biol. 8, 669.
      VanDenmark, A.P., et al. 2001. Cell 105, 711.
      Hofmann, R.M., et al. 1999. Cell96, 645.
      Yamaguchi, T., et al. 1999. J. Biochem.120, 494.
      Product Information
      FormLiquid
      FormulationIn PBS, 1 mM DTT, 10% glycerol, pH 7.2.
      Applications
      Biological Information
      Purity≥95% by SDS-PAGE
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      662103 0

      Documentation

      References

      Reference overview
      McKenna, S., et al. 2003. Biochem. 42, 7922.
      Hofmann, R.M., and Pickart C.M. 2001. J. Biol. Chem. 276, 27936.
      McKenna, S., et al. 2001. J. Biol. Chem. 276, 40120.
      Moraes, T.F., et al. 2001. Nat. Struc. Biol. 8, 669.
      VanDenmark, A.P., et al. 2001. Cell 105, 711.
      Hofmann, R.M., et al. 1999. Cell96, 645.
      Yamaguchi, T., et al. 1999. J. Biochem.120, 494.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision31-May-2009 JSW
      SynonymsUbc13, UBE2N, Ubiquitin-conjugating enzyme E2 N
      DescriptionFull-length, recombinant, human UbcH13 fused at the N-terminus to a His•Tag® sequence and expressed in E. coli. UbcH13, an E2 ubiquitin ligase, forms a heterodimeric complex with Uev1, and is able to assemble K63-linked polyubiquitin chains, which have novel regulatory roles. These ubiquitin chains have a role in a variety of processes such as DNA repair, endocytosis, polysome stability, and signal transduction pathways. The active enzyme is useful in the study of ubiquitin regulation and inhibitor screening.
      FormLiquid
      FormulationIn PBS, 1 mM DTT, 10% glycerol, pH 7.2.
      Concentration Label Please refer to vial label for lot-specific concentration
      Recommended reaction conditionsTypical concentration for in vitro conjugation reactions: 100 nM to 1 µM depending on conditions
      Purity≥95% by SDS-PAGE
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°).
      Toxicity Standard Handling
      ReferencesMcKenna, S., et al. 2003. Biochem. 42, 7922.
      Hofmann, R.M., and Pickart C.M. 2001. J. Biol. Chem. 276, 27936.
      McKenna, S., et al. 2001. J. Biol. Chem. 276, 40120.
      Moraes, T.F., et al. 2001. Nat. Struc. Biol. 8, 669.
      VanDenmark, A.P., et al. 2001. Cell 105, 711.
      Hofmann, R.M., et al. 1999. Cell96, 645.
      Yamaguchi, T., et al. 1999. J. Biochem.120, 494.