OP171
Anti-CD63 Mouse mAb (NKI/C-3)
antibody form
purified antibody
clone
NKI/C-3, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human
isotype
IgG1
Analysis Note
Positive Control
Melanoma tissue
Melanoma tissue
Application
Immunoblotting (see application references)
Paraffin Sections (0.2-0.4 μg/ml, heat pre-treatment required; see application references)
Paraffin Sections (0.2-0.4 μg/ml, heat pre-treatment required; see application references)
General description
Anti-CD63, mouse monoclonal, clone NKI/C-3, recognizes the heterogeneous 25-110 kDa CD63 in melanomas, clear cell sarcomas, and normal melanocytes. It is validated for WB, & IHC on paraffin sections.
CD63 is a glycosyl phosphatidyl inositol (GPI)-anchored, granulocyte-specific activation antigen whose expression is up-regulated in activated neutrophils. It exists as a heterogeneous 25-110 kDa glycoprotein that is located mainly in the innerside of membranes of cytoplasmic vesicles in melanoma cells. The unglycosylated 25 kDa precursor can be detected in tissue sections by immunohistochemistry. CD63 can be detected in melanomas, clear cell sarcomas (melanoma of soft tissue), nevocellular nevi, and normal melanocytes. It has also been detected in some mucus producing tumors, carcinoids, carcinomas of the thyroid, mast cells, histiocytes in tumor regions, and in secretory cells such as salivary glands, bronchial glands, sweat glands, pancreas, and prostate.
Protein G purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with mouse myeloma P3X62Ag8 cells. Recognizes the heterogeneous ~25-110 kDa CD63 glycoprotein.
Recognizes the heterogeneous 25-110 kDa CD63 glycoprotein in melanomas, clear cell sarcomas, nevocellular nevi, and normal melanocytes. Also reacts with some mucous-producing tumors, carcinoids, carcinomas of the thyroid, mast cells, histiocytes in tumor regions, and cells with secretory function.
Immunogen
plasma membrane fraction of human MeWo cells
Other Notes
Exhibits cytoplasmic staining when used in immunohistochemistry. Staining of formalin-fixed, paraffin-embedded tissue sections requires boiling in 10 mM citrate buffer, pH 6.0, for 10-20 min, followed by cooling at room temperature for 20 min. Antibody sh
Physical form
In 10 mM PBS, 0.2% BSA, pH 7.4.
Storage Class
10-13 - German Storage Class 10 to 13
Certificates of Analysis (COA)
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C Vennegoor et al.
Cancer immunology, immunotherapy : CII, 23(2), 93-100 (1986-01-01)
NKI/C-3 and NKI/black-13 are monoclonal antibodies recognizing different epitopes on a melanoma-associated antigen that is preserved after fixation in formalin and embedding in paraffin in virtually all melanoma tissues. The antigen, a predominantly cytoplasmic vesicle membrane-bound heterogeneous glycoprotein of 25-110
A A Palmer et al.
Pathology, 17(2), 335-339 (1985-04-01)
Four methods were compared for identifying amelanotic and oligomelanotic melanomas in paraffin sections of formalin-fixed metastases from subjects with primary cutaneous melanomas. Of the amelanotic and oligomelanotic metastases a characteristic pattern of fluorescence was seen with an incident-light fluorescence microscope
E C Hagen et al.
Histopathology, 10(7), 689-700 (1986-07-01)
In order to investigate their possible role as prognostic markers, staining with the antibodies NKI/C-3 and anti-S100, that are applicable on paraffin sections, was examined using a group of primary cutaneous melanomas and autologous metastases using the immunoperoxidase procedure. All
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