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CP13L Anti-Fibronectin (Ab-1) Mouse mAb (N-294)

CP13L
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      M
      Description
      Overview

      This product has been discontinued.



      Please refer to our complete listing of fibronectin antibodies for possible alternatives.






      Recognizes fibronectin in HT1080 and HS27 cells and normal skin tissue.
      Catalogue NumberCP13L
      Brand Family Calbiochem®
      References
      ReferencesYamada, K.M. 1989. In Fibronectin, Mosher, D.F., ed., 47.
      Rouslahti, E. and Pierschbacher, M.D., 1986. Cell 44, 517.
      Tamkun, J.W., et al. 1986. Cell 46, 271.
      Hynes, R.O. 1985. Cell. Biol. 1, 67.
      Pytela, R., et al. 1985. Cell 40, 191.
      Product Information
      FormLyophilized
      FormulationLyophilized from a volatile buffer, 100 µg BSA.
      Positive controlHT-1080 or HS27 cells or normal skin tissue
      PreservativeNone
      Applications
      Application References Original Clone, Cell Inhibition Assays McDonald, J.A., et al. 1987 J. Biol. Chem. 262, 2957.
      Key Applications Immunofluorescence
      Paraffin Sections
      Cell Inhibition Assays
      Frozen Sections
      Immunoblotting (Western Blotting)
      Application NotesCell Inhibition Assays (see application references)
      Frozen Sections (1-10 µg/ml)
      Immunoblotting (1-5 µg/ml)
      Immunofluorescence (1-5 µg/ml)
      Paraffin Sections (1-10 µg/ml, saponin pre-treatment required)
      Application CommentsAntibody should be titrated for optimal results in individual sytems.
      Biological Information
      Immunogenhuman plasma fibronectin
      ImmunogenHuman
      Epitopewithin the central domain
      CloneN-294
      HostMouse
      IsotypeIgG₁
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsWe recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Fibronectin (Ab-1) Mouse mAb (N-294) Certificates of Analysis

      TitleLot Number
      CP13L

      References

      Reference overview
      Yamada, K.M. 1989. In Fibronectin, Mosher, D.F., ed., 47.
      Rouslahti, E. and Pierschbacher, M.D., 1986. Cell 44, 517.
      Tamkun, J.W., et al. 1986. Cell 46, 271.
      Hynes, R.O. 1985. Cell. Biol. 1, 67.
      Pytela, R., et al. 1985. Cell 40, 191.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision21-August-2007 RFH
      ApplicationCell Inhibition Assays (see application references)
      Frozen Sections (1-10 µg/ml)
      Immunoblotting (1-5 µg/ml)
      Immunofluorescence (1-5 µg/ml)
      Paraffin Sections (1-10 µg/ml, saponin pre-treatment required)
      DescriptionMouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with P3 X 63-Ag8.653 mouse myeloma cells (see application references). Recognizes the fibronectin protein.
      BackgroundFibronectins are a family of plasma and extracellular matrix glycoproteins that contain multiple sites for various ligands and cells. The region of fibronectin that mediates cell adhesion in a majority of cell types is in the carboxy terminal domain and involves the binding of the Arg-Gly-Asp (RGD) sequence to a specific polypeptide cell surface receptor. Although the mechanisms of fibronectin extracellular matrix assembly are not well understood, specific antibodies have been used to determine the domains involved in matrix assembly.
      HostMouse
      Immunogen speciesHuman
      Immunogenhuman plasma fibronectin
      Epitopewithin the central domain
      CloneN-294
      IsotypeIgG₁
      Specieshuman
      Positive controlHT-1080 or HS27 cells or normal skin tissue
      FormLyophilized
      FormulationLyophilized from a volatile buffer, 100 µg BSA.
      PreservativeNone
      CommentsAntibody should be titrated for optimal results in individual sytems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsWe recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesYamada, K.M. 1989. In Fibronectin, Mosher, D.F., ed., 47.
      Rouslahti, E. and Pierschbacher, M.D., 1986. Cell 44, 517.
      Tamkun, J.W., et al. 1986. Cell 46, 271.
      Hynes, R.O. 1985. Cell. Biol. 1, 67.
      Pytela, R., et al. 1985. Cell 40, 191.
      Application references Original Clone, Cell Inhibition Assays McDonald, J.A., et al. 1987 J. Biol. Chem. 262, 2957.