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CB1021 Anti-GMNN Mouse mAb (1A8)

CB1021
  
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      Overview

      Replacement Information

      Key Spec Table

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      Description
      Overview

      This product has been discontinued.





      Recognizes the GMNN protein in human lymph node and placenta tissue.
      Catalogue NumberCB1021
      Brand Family Calbiochem®
      SynonymsAnti-Geminin DNA Replication Imhibitor
      Application Data
      Detection of human GMNN by immunoblotting. Sample: Whole cell lysate from 293T cells transfected with full-length GMNN (lane 1) and from non-transfected 293T cells (lane 2). Primary antibody: Anti-GMNN Mouse mAb (1A8) (Cat. No. CB1021) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human GMNN by staining paraffin sections. Sample: Formalin-fixed paraffin-embedded human lymph node tissue. Primary antibody: Anti-GMNN Mouse mAb (1A8) (Cat. No. CB1021) (3 µg/ml). Detection: colorimetric (DAB).

      Detection of human GMNN by staining paraffin sections. Sample: Formalin-fixed paraffin-embedded human placenta tissue. Primary antibody: Anti-GMNN Mouse mAb (1A8) (Cat. No. CB1021) (1 µg/ml). Detection: colorimetric (DAB).
      References
      Product Information
      Formliquid
      FormulationIn PBS, pH 7.2.
      Negative control293T cells
      Positive controlHuman lymph node tissue, Human placenta tissue
      PreservativeNone
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids 110-209 of human GMNN, expressed as a GST fusion protein
      ImmunogenNA
      Clone1A8
      HostMouse
      IsotypeIgG₁
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision29-October-2010 RFH
      SynonymsAnti-Geminin DNA Replication Imhibitor
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application Data
      Detection of human GMNN by immunoblotting. Sample: Whole cell lysate from 293T cells transfected with full-length GMNN (lane 1) and from non-transfected 293T cells (lane 2). Primary antibody: Anti-GMNN Mouse mAb (1A8) (Cat. No. CB1021) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human GMNN by staining paraffin sections. Sample: Formalin-fixed paraffin-embedded human lymph node tissue. Primary antibody: Anti-GMNN Mouse mAb (1A8) (Cat. No. CB1021) (3 µg/ml). Detection: colorimetric (DAB).

      Detection of human GMNN by staining paraffin sections. Sample: Formalin-fixed paraffin-embedded human placenta tissue. Primary antibody: Anti-GMNN Mouse mAb (1A8) (Cat. No. CB1021) (1 µg/ml). Detection: colorimetric (DAB).
      DescriptionPurified mouse monoclonal antibody. Recognizes the GMNN protein.
      BackgroundGMNN inhibits DNA replication by preventing the incorporation of MCM complex into prereplication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle.
      HostMouse
      Immunogen speciesNA
      ImmunogenA recombinant polypeptide corresponding to amino acids 110-209 of human GMNN, expressed as a GST fusion protein
      Clone1A8
      IsotypeIgG₁
      Specieshuman
      Positive controlHuman lymph node tissue, Human placenta tissue
      Negative control293T cells
      Formliquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review