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CB1029 Anti-HAMP Mouse mAb (1F9)

CB1029
  
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      Overview

      Replacement Information

      Key Spec Table

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      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the HAMP protein.
      Catalogue NumberCB1029
      Brand Family Calbiochem®
      SynonymsAnti-Hepcidin Antimicrobial Peptide
      Application Data
      Detection of human HAMP by immunoblotting. Sample: Partial recombinant HAMP. Primary antibody: Anti-HAMP Mouse mAb (1F9) (Cat. No. CB1029) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human HAMP by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human liver tissue. Primary antibody: Anti-HAMP Mouse mAb (1F9) (Cat. No. CB1029) (3 µg/ml). Detection: colorimentric (DAB).
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Positive controlHuman liver tissue
      PreservativeNone
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids 25-85 of human HAMP, expressed as a GST fusion protein
      ImmunogenNA
      Clone1F9
      HostMouse
      IsotypeIgG₁
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision29-October-2010 RFH
      SynonymsAnti-Hepcidin Antimicrobial Peptide
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application Data
      Detection of human HAMP by immunoblotting. Sample: Partial recombinant HAMP. Primary antibody: Anti-HAMP Mouse mAb (1F9) (Cat. No. CB1029) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human HAMP by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human liver tissue. Primary antibody: Anti-HAMP Mouse mAb (1F9) (Cat. No. CB1029) (3 µg/ml). Detection: colorimentric (DAB).
      DescriptionPurified mouse monoclonal antibody. Recognizes the HAMP protein.
      BackgroundThe protein is involved in the maintenance of iron homeostasis, and it is necessary for the regulation of iron storage in macrophages, and for intestinal iron absorption. The preproprotein is post-translationally cleaved into mature peptides of 20, 22 and 25 amino acids, and these active peptides are rich in cysteines, which form intramolecular bonds that stabilize their beta-sheet structures. Mutations in this protein cause hemochromatosis type 2B, also known as juvenile hemochromatosis, a disease caused by severe iron overload that result in cardiomyopathy, cirrhosis, and endocrine failure.
      HostMouse
      Immunogen speciesNA
      ImmunogenA recombinant polypeptide corresponding to amino acids 25-85 of human HAMP, expressed as a GST fusion protein
      Clone1F9
      IsotypeIgG₁
      Specieshuman
      Positive controlHuman liver tissue
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review