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PC255L Anti-Parvalbumin Rabbit pAb

PC255L
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      H, M, R Rb Polyclonal Antibody
      Description
      OverviewRecognizes the parvalbumin protein in rat muscle, thalamus, hippocampus, and cortex tissue.
      Catalogue NumberPC255L
      Brand Family Calbiochem®
      References
      ReferencesHeizmann, C.W. 1993. Acta Neurobiol. Exp. 53, 15-23.
      Baimbridge, K.G., et al. 1992. Trends Neurosci. 15, 303.
      Heizmann, C.W. and Hunziker, W. 1992. Trends Biochem. Sci. 16, 98.
      Product Information
      FormLyophilized
      FormulationUndiluted serum.
      Positive controlRat muscle, rat thalamus, hippocampus or cortex tissue
      Preservative≤0.1% sodium azide
      Applications
      Application ReferencesSpecificity Mithani, S., et al. 1987. Psychopharmacology 93, 94.
      Key Applications Frozen Sections
      Immunofluorescence
      T Cell Co-stimulation
      Application NotesFrozen Sections (1:4000-1:8000 for biotin-streptavidin/ peroxidase detection)
      Immunofluorescence (1:400-1:800, indirect)
      Vibrotome Sections (1:4000-1:8000 for biotin-streptavidin/ peroxidase detection)
      Application CommentsAntibody specificity was examined in the rat thalamus, hippocampus, and cortex (see application references). Detection of primary antibody with biotin-streptavidin/peroxidase reagents according to manufacturers directions. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenpurified rat muscle paravalbumin
      ImmunogenRat
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsReconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Resulting reconstituted solution will contain ≤0.1% sodium azide and 1% BSA. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Parvalbumin Rabbit pAb MSDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Parvalbumin Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC255L

      References

      Reference overview
      Heizmann, C.W. 1993. Acta Neurobiol. Exp. 53, 15-23.
      Baimbridge, K.G., et al. 1992. Trends Neurosci. 15, 303.
      Heizmann, C.W. and Hunziker, W. 1992. Trends Biochem. Sci. 16, 98.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision09-October-2007 RFH
      ApplicationFrozen Sections (1:4000-1:8000 for biotin-streptavidin/ peroxidase detection)
      Immunofluorescence (1:400-1:800, indirect)
      Vibrotome Sections (1:4000-1:8000 for biotin-streptavidin/ peroxidase detection)
      DescriptionRabbit polyclonal antibody supplied as lyophilized undiluted serum. Recognizes the parvalbumin protein.
      BackgroundSeveral EF-hand calcium-binding proteins have been identified within the nervous system, which includes calbindin, calretinin, and parvalbumin. These cytosolic calcium-binding proteins modulate and mediate the function of the intracellular second messenger calcium. These proteins are believed to function in the buffering, transport and regulation of calcium. Calbindin, calretinin, and parvalbumin are localized in distinct neuronal subpopulations, and antibodies against these proteins are useful markers for neuranatomical and developmental studies.
      HostRabbit
      Immunogen speciesRat
      Immunogenpurified rat muscle paravalbumin
      IsotypeIgG
      Specieshuman, mouse, rat
      Positive controlRat muscle, rat thalamus, hippocampus or cortex tissue
      FormLyophilized
      FormulationUndiluted serum.
      Preservative≤0.1% sodium azide
      CommentsAntibody specificity was examined in the rat thalamus, hippocampus, and cortex (see application references). Detection of primary antibody with biotin-streptavidin/peroxidase reagents according to manufacturers directions. Antibody should be titrated for optimal results in individual systems.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Resulting reconstituted solution will contain ≤0.1% sodium azide and 1% BSA. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.
      Toxicity Standard Handling
      ReferencesHeizmann, C.W. 1993. Acta Neurobiol. Exp. 53, 15-23.
      Baimbridge, K.G., et al. 1992. Trends Neurosci. 15, 303.
      Heizmann, C.W. and Hunziker, W. 1992. Trends Biochem. Sci. 16, 98.
      Application referencesSpecificity Mithani, S., et al. 1987. Psychopharmacology 93, 94.