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PC347 Anti-Serotonin 3 (5-HT₃) Receptor (444-457) Rabbit pAb

PC347
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
M, R Rb Polyclonal Antibody

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PC347-25UG
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      Plastic ampoule 25 μg
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      Description
      OverviewRecognizes the serotonin 3 (5-HT3) receptor in mouse and rat brain tissue.
      Catalogue NumberPC347
      Brand Family Calbiochem®
      Application Data
      Detection of human Serotonin 5-HT3 Receptor by staining frozen sections. Samples: Floating mouse brain fixed in 4% paraformaldehyde. Primary antibody: Anti-Serotonin (5-HT3) Receptor (444-457) Rabbit pAb (Cat. No. PC347) (5 µg/ml). Detection: DAB.
      References
      ReferencesMorales, M., et al. 1998. J. Comp. Neurol. 385, 385.
      Morales, M., et al. 1996. Brain Res. 731, 199.
      Morales, M., et al. 1996. Mol. Brain Res. 36, 251.
      Teitler, M. and Herrick-Davis, K. 1994. Crit. Rev. Neurobiol. 8, 175.
      Tecott, L.H., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 1430.
      Maricq, A.V., et al. 1991. Science 254, 432.
      Pratt, G.D., et al. 1990. Trends Pharmacol. Sci. 11, 135.
      Derkach, V., et al. 1989. Nature 33, 706.
      Product Information
      FormLiquid
      Formulation25 µg antibody in 0.05 M sodium phosphate buffer, 0.2% gelatin
      Positive controlMouse or rat brain tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Frozen Sections
      Not Western Blot
      Application NotesFrozen Sections (2-5 µg/ml)
      Immunoblotting (not recommended)
      Application CommentsThis antibody is not recommended for immunoblotting using total mouse or rat brain extract. Immunohistochemistry was performed using floating mouse and rat brain sections fixed in 4% paraformaldehyde. Staining was completely abolished by preincubating the affinity purified antibody with control peptide at 10-6M. Unconjugated serotonin 5-HT3 receptor control peptide has been included for use as an internal control for antibody specificity. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide (SLEKRDEMREVARD) corresponding to amino acids 444-457 of rat serotonin (5-HT₃) receptor, conjugated to KLH
      ImmunogenRat
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Serotonin 3 (5-HT₃) Receptor (444-457) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC347

      References

      Reference overview
      Morales, M., et al. 1998. J. Comp. Neurol. 385, 385.
      Morales, M., et al. 1996. Brain Res. 731, 199.
      Morales, M., et al. 1996. Mol. Brain Res. 36, 251.
      Teitler, M. and Herrick-Davis, K. 1994. Crit. Rev. Neurobiol. 8, 175.
      Tecott, L.H., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 1430.
      Maricq, A.V., et al. 1991. Science 254, 432.
      Pratt, G.D., et al. 1990. Trends Pharmacol. Sci. 11, 135.
      Derkach, V., et al. 1989. Nature 33, 706.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision03-October-2016 JSW
      ApplicationFrozen Sections (2-5 µg/ml)
      Immunoblotting (not recommended)
      Application Data
      Detection of human Serotonin 5-HT3 Receptor by staining frozen sections. Samples: Floating mouse brain fixed in 4% paraformaldehyde. Primary antibody: Anti-Serotonin (5-HT3) Receptor (444-457) Rabbit pAb (Cat. No. PC347) (5 µg/ml). Detection: DAB.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the serotonin (5-HT3) receptor.
      BackgroundThe monoamine serotonin (5-hydroxytryptamine) mediates a variety of physiological actions by binding to a number of distinct receptors, pharmacologically classified into several major groups which differ in tissue and cellular localization, affinity for serotonin, and second messenger pathways. The majority of these receptors stimulate a GTP-binding protein upon agonist stimulation and couple to adenylate cyclase or phospholipase C. In contrast, the 5-HT3 receptor is a ligand-gated ion channel that mediates a fast excitatory response. This receptor is a member of the superfamily of ligand-gated ion channels related to the nicotinic, GABAA and NMDA receptors. Although the 5-HT3 receptor was initially identified from the neuroblastoma cell line NCB-20, radioligand binding studies and in situ hybridization studies have localized the 5-HT3 receptor to a unique distribution of neurons, supporting possible roles for the receptor in cognition and sensory input. Recent immunohistochemical analysis of the rat brain revealed a broad distribution of 5-HT3 immunoreactivity, primarily including interneurons and motor neurons.
      HostRabbit
      Immunogen speciesRat
      Immunogena synthetic peptide (SLEKRDEMREVARD) corresponding to amino acids 444-457 of rat serotonin (5-HT₃) receptor, conjugated to KLH
      IsotypeIgG
      Speciesmouse, rat
      Positive controlMouse or rat brain tissue
      FormLiquid
      Formulation25 µg antibody in 0.05 M sodium phosphate buffer, 0.2% gelatin
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsThis antibody is not recommended for immunoblotting using total mouse or rat brain extract. Immunohistochemistry was performed using floating mouse and rat brain sections fixed in 4% paraformaldehyde. Staining was completely abolished by preincubating the affinity purified antibody with control peptide at 10-6M. Unconjugated serotonin 5-HT3 receptor control peptide has been included for use as an internal control for antibody specificity. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesMorales, M., et al. 1998. J. Comp. Neurol. 385, 385.
      Morales, M., et al. 1996. Brain Res. 731, 199.
      Morales, M., et al. 1996. Mol. Brain Res. 36, 251.
      Teitler, M. and Herrick-Davis, K. 1994. Crit. Rev. Neurobiol. 8, 175.
      Tecott, L.H., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 1430.
      Maricq, A.V., et al. 1991. Science 254, 432.
      Pratt, G.D., et al. 1990. Trends Pharmacol. Sci. 11, 135.
      Derkach, V., et al. 1989. Nature 33, 706.