Millipore Sigma Vibrant Logo

71855 Gateway® Nova pCOLA-2-DEST™ DNA - Novagen

71855
  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      The Gateway® Nova pCOLA-2-DEST™ vector carries the COLA replicon from ColA and the kanamycin resistance gene. This DEST vector is compatible with pENTR vectors created using pDONR223, the pDONR vector used in the human ORFeome project. This DEST vector is NOT compatible with pENTR vectors derived from pDONR221 or other pENTR vectors carrying the kanR gene on their backbone. pEXPR recombinants created using pCOLA-2-DEST can be used for coexpression with expression plasmids generated from Gateway Nova pET-DEST vectors carrying the ColE1 replicon and AmpR gene. pCOLA-2-DEST is designed to create expression clones (pEXPR) that are fused to an N-terminal His•Tag® coding sequence. If the pENTR clone lacks a stop codon and is appropriately designed for a C-terminal fusion, the target gene in the resulting pEXPR clone will also be fused to a C-terminal Strep•Tag® II coding sequence. The presence of a "gentle elution" tag at each terminus is ideal for dual purification strategies designed to isolate full-length fusion proteins.




      This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
      Catalogue Number71855
      Brand Family Novagen®
      References
      Product Information
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage ≤ -70°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications