Millipore Sigma Vibrant Logo

539663 HtrA2/Omi, Human, Recombinant, E. coli

539663
  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Spec Table

      Description
      Overview

      This product has been discontinued.





      Recombinant human HtrA2/Omi (amino acids 134-458) fused at the C-terminus to a 6X His•Tag® sequence and expressed in E. coli. HtrA2/Omi is a mitochondrial trypsin-like serine protease that is processed to expose an amino-terminal Reaper-like motif similar to SMAC/Diablo. It is released from the mitochondria in response to apoptotic stimuli and can interact with the BIR2 or BIR3 domains of XIAP to reverse caspase inhibition. The specificity of the protease activity has not been determined and there are currently no identified endogenous substrates.
      Catalogue Number539663
      Brand Family Calbiochem®
      References
      ReferencesMartins, L., et al. 2002. J. Biol. Chem. 277, 439.
      Silke, J. and Verhagen, A. 2002. Cell Death. Differ. 9, 362.
      van Loo, G., et al. 2002. Cell Death. Differ. 9, 20.
      Hedge, R. et al. 2001. J. Biol. Chem. 277, 432.
      Suzuki, Y., et al. 2001. Mol. Cell. 8, 613.
      Verhagen, A., et al. 2001. J. Biol. Chem. 277, 445.
      Savopoulos, J., et al. 2000. Protein Expr. Purif.19, 227.
      Product Information
      FormLiquid
      FormulationIn sterile-filtered 100 mM NaCl, 25 mM HEPES, 1 mM DTT, 20% glycerol, pH 8.0.
      Applications
      Biological Information
      Biological activityReversal of XIAP-BIR2-mediated caspase-7 inhibition (50%) = 0.2-1.5 µM as determined by an increase in the rate of DEVD-AFC cleavage by 2 ng caspase-7 in the presence of XIAP-BIR2. Protease activity: 0.2-1.0 nmol HtrA2/0mi will clease ~50% OF 1 µg β-casein at 37°C for 2 h as monitored by SDS-PAGE. Protease activity: 0.2-1.0 nmol HtrA2/Omi will cleave ~50% of 1 µg β-casein at 37°C for 2 h as monitored by SDS-PAGE.
      Purity≥85% by SDS-PAGE
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      HtrA2/Omi, Human, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      539663

      References

      Reference overview
      Martins, L., et al. 2002. J. Biol. Chem. 277, 439.
      Silke, J. and Verhagen, A. 2002. Cell Death. Differ. 9, 362.
      van Loo, G., et al. 2002. Cell Death. Differ. 9, 20.
      Hedge, R. et al. 2001. J. Biol. Chem. 277, 432.
      Suzuki, Y., et al. 2001. Mol. Cell. 8, 613.
      Verhagen, A., et al. 2001. J. Biol. Chem. 277, 445.
      Savopoulos, J., et al. 2000. Protein Expr. Purif.19, 227.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision02-September-2009 JSW
      DescriptionRecombinant human HtrA2/Omi (amino acids 134-458) fused at the C-terminus to a 6X His•Tag® sequence and expressed in E. coli. HtrA2/Omi is a mitochondrial trypsin-like serine protease that is processed to expose an amino-terminal Reaper-like motif similar to SMAC/Diablo. It is released from the mitochondria in response to apoptotic stimuli and can interact with the BIR2 or BIR3 domains of XIAP to reverse caspase inhibition. The specificity of the protease activity has not been determined and there are currently no identified endogenous substrates.
      FormLiquid
      FormulationIn sterile-filtered 100 mM NaCl, 25 mM HEPES, 1 mM DTT, 20% glycerol, pH 8.0.
      Concentration Label Please refer to vial label for lot-specific concentration
      Purity≥85% by SDS-PAGE
      Biological activityReversal of XIAP-BIR2-mediated caspase-7 inhibition (50%) = 0.2-1.5 µM as determined by an increase in the rate of DEVD-AFC cleavage by 2 ng caspase-7 in the presence of XIAP-BIR2. Protease activity: 0.2-1.0 nmol HtrA2/0mi will clease ~50% OF 1 µg β-casein at 37°C for 2 h as monitored by SDS-PAGE. Protease activity: 0.2-1.0 nmol HtrA2/Omi will cleave ~50% of 1 µg β-casein at 37°C for 2 h as monitored by SDS-PAGE.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesMartins, L., et al. 2002. J. Biol. Chem. 277, 439.
      Silke, J. and Verhagen, A. 2002. Cell Death. Differ. 9, 362.
      van Loo, G., et al. 2002. Cell Death. Differ. 9, 20.
      Hedge, R. et al. 2001. J. Biol. Chem. 277, 432.
      Suzuki, Y., et al. 2001. Mol. Cell. 8, 613.
      Verhagen, A., et al. 2001. J. Biol. Chem. 277, 445.
      Savopoulos, J., et al. 2000. Protein Expr. Purif.19, 227.