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EAMT003 PDH ELISA Kit

EAMT003
  
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      Overview

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      Overview

      This product has been discontinued.





      Product Description

      The Pyruvate Dehydrogenase (PDH) ELISA Kit can be used to determine the amount of PDH protein in a sample. This assay is a 'sandwich' ELISA, where the PDH enzyme is purified and immobilized by an anti-PDH capture antibody pre-coated in the microplate wells. The amount of captured PDH is determined by adding a second (detector) anti-PDH antibody which binds to the captured PDH, followed by binding of an HRP conjugated goat anti-mouse antibody that binds the detector anti-PDH antibody. The detector-bound HRP then changes the colorless HRP development solution to blue and the color intensity (absorbance) is proportional to the amount of PDH captured.



      Panel Components

      20 ml 	20X Buffer, (72252-20ML)
      1 ml	Detergent, (72253-1ML)
      1 ml	20X Detector Antibody, (72254-1ML)
      1 ml	20 HRP Label, (72255-1ML)
      20 ml	HRP Development Solution, (72556-20ML)
      1 ea	Microplate, (72257-1EA)
      13 ml	5X Stabilizer, (72258-13ML)
      10 ml	10X Blocking Solution, (72259-10ML)
      

      * This kit contains sufficient materials for 96 measurements (one 96-well microplate).

      Storage: Store the 5X stabilizer at –20°C. Store all other components at 4°C. When stored as recommended, the kit is stable for at least 6 months.

      Note: Avoid freeze-thaw cycles cycles of frozen components by making appropriate aliquots if the entire plate is not run in a single 96-well experiment.



      This kit contains the necessary buffers and reagents for the respective reaction. The kit also contains a 96-well microplate with a monoclonal antibody pre-bound to the wells of the microplate. This plate can be broken into 12 separate 8-well strips for convenience; therefore the plate can be used for up to 12 separate experiments.
      Catalogue NumberEAMT003
      Brand Family Novagen®
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      Ship Code Multiple Storage Conditions
      Toxicity Irritant
      Storage Multiple storage requirements
      Do not freeze Ok to freeze
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