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70049 pBACgus-2cp DNA

70049
  
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      Overview

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      Overview

      This product has been discontinued.





      pBACgus-2cp is a baculovirus transfer plasmid designed for simplified cloning and expression of target genes in insect cells. The plasmid is compatible with BacVector™-1000, -2000 or -3000 Triple Cut Virus DNA for low background transfection and efficient utilization of the polh promoter. The plasmid also carries the gus gene encoding b-glucuronidase under control of the late basic protein promoter (P6.9), which serves as a reporter to verify recombinant viruses by staining with X-gluc. pBACgus-2cp provides an ATG start codon at the optimal position relative to native polyhedrin translation signals. Cloning sites are provided for the creation of N-terminal fusions of an insert with His•Tag® and/or S•Tag™ sequences. A Ligation-Independent Cloning (LIC) version of the vector is available for rapid, directional cloning of PCR products adjacent to the enterokinase cleavage site. The vector provides optional expression of a C-terminal His•Tag fusion sequence by allowing read-through of inserts in the proper reading frame. Unique restriction sites are indicated on the circle map.
      Catalogue Number70049
      Brand Family Novagen®
      References
      Product Information
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      pBACgus-2cp DNA MSDS

      Title

      Safety Data Sheet (SDS) 

      pBACgus-2cp DNA Certificates of Analysis

      TitleLot Number
      70049

      Brochure

      Title
      Insect Cell Expression

      Citations

      Title
    • Carla V. Finkielstein, Michael Overduin and Daniel G. Capelluto. (2006) Cell migration and signaling specificity is determined by Rac1's phosphatidylserine recognition motif. Journal of Biological Chemistry 281, 27317-27326.
    • Vector Map

      Title
      TB141VM pBACgus-2cp Vector Map

      Vector Sequence

      Title
      pBACgus-2cp Sequence

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      Categories

      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Insect Expression > Insect Expression Vectors & Kits