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71241 pIEx™-1 DNA - Novagen

71241
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      Glass bottle 20 μg
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      Description
      OverviewThe pIEx™ vectors are designed for cloning and high-level expression of proteins in transiently transfected Spodoptera-derived insect cells. Transcription is driven by the AcNPV-derived hr5 enhancer and immediate early promoter, IE1. pIEx-1 contains N-terminal His•Tag and S•Tag coding sequences, and a C-terminal HSV•Tag coding sequence. N-terminal vector encoded sequences can be completely removed by cloning into the PshA I site and cleaving the fusion protein with enterokinase.
      pIEx-1 is also available as an Ek/LIC vector. The Ek/LIC vector is prepared for rapid directional cloning of PCR-amplified DNA. Using specifically designed primers for amplification, inserts can be efficiently cloned without the need for restriction enzyme digestion or ligation.

      For sequencing or PCR of recombinants in pIEx-1, refer to the table below to choose the appropriate primers.
      Vector5′ Primer3′ Primeralt 5′ Primer
      pIEx-1S•Tag 18mer PrimerAS HSV•Tag PrimerIE1 Promoter Primer
      pIEx-2S•Tag 18mer PrimerAS HSV•Tag PrimerIE1 Promoter Primer
      pIEx-3S•Tag 18mer PrimerAS HSV•Tag PrimerIE1 Promoter Primer
      pIEx-4IE1 Promoter PrimerAS S•Tag 18mer Primer
      NA
      pIEx-5IE1 Promoter PrimerAS S•Tag 18mer Primer
      NA
      pBiEx-1S•Tag 18mer PrimerAS HSV•Tag PrimerIE1 Promoter Primer
      T7 Promoter Primer
      pBiEx-2S•Tag 18mer PrimerAS HSV•Tag PrimerIE1 Promoter Primer
      T7 Promoter Primer
      pBiEx-3IE1 Promoter PrimerAS S•Tag 18mer PrimerT7 Promoter Primer





      This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
      Catalogue Number71241
      Brand Family Novagen®
      SynonymsInsectDirect™ System- pIEx™-1 DNA
      References
      Product Information
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      pIEx™-1 DNA - Novagen Certificates of Analysis

      TitleLot Number
      71241

      Brochure

      Title
      Insect Cell Expression
      Insect Directル Protein Expression & Purification System

      Citations

      Title
    • Soo-Mi Kim, et al. (2005) Phosphorylation of Chk1 by ATM- and Rad3-related (ATR) in Xenopus egg extracts requires binding of ATRIP to ATR but not the stable DNA-binding or coiled-coil domains of ATRIP. Journal of Biological Chemistry 280, 38355-38364.
    • User Protocols

      Title
      TB356 InsectDirect™ pIEx™ Vectors

      Vector Map

      Title
      TB349VM pIEx™-1 Vector Map

      Vector Sequence

      Title
      pIEx™-1 Vector Sequence