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70826 pTriEx™-2 DNA

70826
  
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      Overview

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      Overview

      This product has been discontinued.





      The pTriEx™-2 vector is uniquely designed to allow rapid characterization of target genes in multiple expression systems. With this vector a single recombinant plasmid can be used to test expression in E. coli, insect and vertebrate cells. Transient vertebrate expression is mediated by a hybrid promoter composed of the CMV immediate early enhancer fused to the chicken β-actin promoter. For expression in insect cells, pTriEx-2 contains flanking baculovirus sequences to permit the generation of recombinant baculoviruses using the BacVector™ System. In baculovirus-infected insect cells, expression is driven by the very late p10 promoter. Expression in E. coli is regulated by the tightly controlled T7lac promoter. Expression can be induced in hosts such as NovaBlue by infecting with λCE6, a phage that constitutively expresses T7 RNA polymerase from the λpL and λlpI promoters. Alternatively, pTriEx recombinant plasmids can be transferred into a (DE3)pLacI host that allows IPTG based induction. Native protein can be expressed by cloning into the NcoI site. Alternatively, native protein can be generated by cloning into the PshA I or Sma I sites and cleavage of the fusion protein with Enterokinase or Thrombin, respectively.


      The pTriEx vector is covered by US Patent 6,589,783 owned by EMD Millipore Corporation or its Affiliates. This Product is sold for research use only. Any commercial use of the Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Further, the pTriEx vector allows for expression of target genes under control of the T7 promoter and is also covered by US Patent 5,693,489. This patent is licensed by EMD Millipore Corporation from Brookhaven National Labs for research use only. Commercial entities need to obtain a research use license from Brookhaven National Labs prior to use of this Product. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
      Catalogue Number70826
      Brand Family Novagen®
      References
      Product Information
      DeclarationThis product is not available in Japan.
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      pTriEx™-2 DNA MSDS

      Title

      Safety Data Sheet (SDS) 

      pTriEx™-2 DNA Certificates of Analysis

      TitleLot Number
      70826

      Brochure

      Title
      Insect Cell Expression

      Citations

      Title
    • Trine Nilsen, et al. (2007) A nuclear export sequence located on a β-strand in fibroblast growth factor-1. Journal of Biological Chemistry 282, 26245-26256.
    • Katherine J. Strissel, et al. (2005) Recoverin undergoes light-dependent intracellular translocation in rod photoreceptors. Journal of Biological Chemistry 280, 29250-29255.
    • Jedrzej Malecki, et al. (2004) Translocation of FGF-1 and FGF-2 across vesicular membranes occurs during G1-phase by a common mechanism. Molecular Biology of the Cell 15, 801-814.
    • User Protocols

      Title
      TB053 Academic and Non-profit Laboratory Assurance Letter
      TB250 pTriEx™ System Manual

      Vector Map

      Title
      TB277 pTriEx™-2 Vector Map

      Vector Sequence

      Title
      pTriEx-2 Sequence

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      Categories

      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Insect Expression > Insect Expression Vectors & Kits
      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Mammalian Expression > Mammalian Expression Vectors & Kits
      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors