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03-0136-00 SMC™ Mouse IL-4 Discovery Immunoassay Kit

The Single Molecule Counting (SMC™) Mouse IL-4 Discovery Immunoassay has unparalleled performance in quantifying IL-4 in mouse serum.

SMC™ Mouse IL-4 Discovery Immunoassay Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Single Molecule Counting (SMC®) Research Category: Inflammation & Immunology Research Sub-Category: Cytokines & Cytokine Receptors
03-0136-00
96 well assay  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Analytes AvailableSpecies ReactivityKey Applications
IL-4 MSingle Molecule Counting (SMC®)
Description
Catalogue Number03-0136-00
Trade Name
  • SMC®
DescriptionSMC™ Mouse IL-4 Discovery Immunoassay Kit
OverviewThe SMC™ Mouse IL-4 Discovery Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC™ immunoassay system detects analytes using Single Molecule Counting (SMC™) technology.
Background InformationInterleukin-4 (IL-4) is a cytokine that is a key regulator in humoral and adaptive immunity. IL-4 induces differentiation of naive helper T-cells (Th0 cells) to Th2 cells. It has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. Further, IL-4 controls the production of IgE, expands IL-4 producing T-cell subsets and stabilizes effector cell functions. IL-4 has great therapeutic potential due to its role in the development of allergic inflammatory responses. IL-4 also has shown to have promise in drug targeting for cancer.
References
Product Information
Components
  • Assay Buffer
  • Mouse IL-4 Coating Antibody
  • Assay Plate
  • 10X PBS
  • Blocking Buffer
  • Standard Diluent
  • Mouse IL-4 Detection Antibody
  • Mouse IL-4 Standard
  • SMC™ Mouse IL-4 Immunoassay Kit Instructions
  • 10X Wash Buffer
  • Buffer C
  • Elution Buffer B
Precision, %
  • Low-end CV% Range: 0-20%
  • Low-end CV% Average: 4%
Quality LevelMQ200
Applications
ApplicationThe Single Molecule Counting (SMC™) Mouse IL-4 Discovery Immunoassay has unparalleled performance in quantifying IL-4 in mouse serum.
Key Applications
  • Single Molecule Counting (SMC®)
Biological Information
Species Reactivity
  • Mouse
Analytes Available
  • IL-4
Accuracy
  • Minimum of 10 samples spiked with acceptable recovery between 80-120%.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size96 well assay
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
03-0136-00 04055977317756

Related Products & Applications

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Catalogue Number Description
03-0123-00 SMC™ Mouse IL-17A Discovery Immunoassay Kit
03-0125-01 SMC™ Mouse IL-17F Discovery Immunoassay Kit
03-0134-00 SMC™ Mouse IL-10 Discovery Immunoassay Kit

Categories

Life Science Research > Antibodies and Assays > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits
Life Science Research > Protein Detection and Quantification > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits