Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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Select A Species, Panel Type, Kit or Sample Type
To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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96-Well Plate
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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Parasites are responsible for a number of human diseases that cause significant morbidity and mortality, including malaria, African sleeping sickness, leishmaniasis and Lyme disease. Understanding how parasites infect the body and evade clearance by the host immune response is of key importance to developing effective therapeutics to treat them. Using the image-based approach provided by Amnis® imaging flow cytometry, it is possible to not only detect the population of infected cells but also determine the number of parasites residing within cells, as well as their impact on sub-cellular signaling events.
Trypanosoma brucei is a parasite which causes African sleeping sickness. Shown in the figure is a study in which nuclear localization of a GFP tagged molecule was quantitated in two samples. The histogram shows the Similarity score for a cross correlation between the GFP image and the DRAQ5 nuclear image for each cell. A high Similarity score indicates that the GFP image and the DRAQ5 image are highly similar (GFP has translocated to the nucleus). A low Similarity score is obtained for untranslocated events. See the application note for more details.
Babesia Spot Count Within RBC
Babesiosis is a malaria-like disease caused by infection of red blood cells by the parasite Babesia. While healthy individuals may be asymptomatic, infection can be fatal in immune-compromised people. Using the ImageStream, Babesia were identified within infected RBC by staining parasite DNA with YOYO1. The stage of infection can be inferred by counting the number of infected cells and the number of parasites in each RBC.