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ABN2192-25UL Anti-Calbindin D-28K

Anti-Calbindin D-28K Antibody, Cat. No. ABN2192, is a highly specific rabbit polyclonal antibody that targets Calbindin D and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Anti-Calbindin D-28K MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: Calb1 UniProt Number: P12658
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ABN2192-25UL
25 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, H, RICC, IH(P), WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN2192-25UL
      DescriptionAnti-Calbindin D-28K
      Alternate Names
      • Calbindin
      • Calbindin D28
      • D-28K
      • PCD-29
      • Spot 35 protein
      • Vitamin D-dependent calcium-binding protein
      Background InformationCalbindin (UniProt: P12658; also known as Calbindin D28, D-28K, PCD-29, Spot 35 protein, Vitamin D-dependent calcium-binding protein, avian-type) is encoded by the calb1 gene (gene ID: 12307) in murine species. Calbindin D-28k is a member of a large family of intracellular calcium-binding proteins of the EF-hand related to calmodulin and troponin-C. Calbindin D-28K has four functional calcium-binding sites (aa 24-35; 111-122; 155-166; and 199-210). It has moderate cytoplasmic mobility and comprises six EF-hands, of which one or two are non-functioning in metal binding. EF-2 is viewed as non-metal binding and EF-6 may have a very low Ca2+ affinity. Four EF hands function in calcium binding with rapid to intermediate kinetics and affinity. The biological roles of Calbindin D-28K include calcium regulation and signaling, including calcium transport and uptake, calcification of bone and teeth, and calcium related signaling in neurons and transiently in embryological development. It also protects neurons from apoptotic cell death. Decline in Calbindin D-28K levels in hippocampal dentate granule cells correlates with the kindling model for epilepsy. Calbindin D-28K immunoreactivity is shown to be specifically absent from neuronal populations lost early in ALS, while substantial loss of neurons, which do contain this protein occur in patients with Huntington's disease. (Ref.: Schmidt, H (2012). Front. Mol. Neurosci. 5; 25).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Calbindin D-28K Antibody, Cat. No. ABN2192, is a highly specific rabbit polyclonal antibody that targets Calbindin D and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Calbindin D-28K in E18 rat cortical cells.

      Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Calbindin D-28K in mouse kidney and human cerebellum tissue sections.
      Biological Information
      ImmunogenGST/His-tagged recombinant fragment corresponding to 107 amino acids from the C-terminal end of murine Calbindin D-28K.
      EpitopeC-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Calbindin D-28K in Human, Murine, and Rat cells. It targets an epitope within 107 amino acids from the C-terminal region.
      Species Reactivity
      • Mouse
      • Human
      • Rat
      Species Reactivity NoteHuman, Mouse, Rat.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Calb1
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~28 kDa observed; 29.99 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human brain tissue lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected Calbindin D-28K in human brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABN2192-25UL 04054839448065