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324870 Anti-α-Epithelial Cell Sodium Channel (20-42) Rabbit pAb

This Anti-α-Epithelial Cell Sodium Channel (20-42) Rabbit pAb is validated for use in Immunoblotting, Immunoprecipitation for the detection of α-Epithelial Cell Sodium Channel (20-42).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-α-ENaC

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324870
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, RRbPolyclonal Antibody

Pricing & Availability

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324870-100UGCN
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      Description
      OverviewRecognizes the ~97 kDa glycosylated α-ENaC protein.
      Catalogue Number324870
      Brand Family Calbiochem®
      SynonymsAnti-α-ENaC
      References
      ReferencesBenos, D.J., and Stanton, B.A. 1999. J. Physiol. London 520, 631.
      Mano, I., and Driscoll, M. 1999. Bioessays 21, 568.
      Fyfe, G.K., et al. 1998. Semin. Nephrol. 18, 138.
      Product Information
      FormLiquid
      FormulationIn PBS, 1 mg/ml BSA.
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesImmunoblotting (1 µg/ml)
      Immunoprecipitation (see comments)
      Application CommentsDoes not cross-react with β-ENaC or γ-ENaC. Also reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.
      Biological Information
      Immunogena synthetic peptide [LMKGNKREEQGLGPEPAAPQQPT(C)] corresponding to amino acids 20-42 of human α-epithelial cell sodium channel (α-ENaC), conjugated to carrier protein
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Rat
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      324870-100UGCN 04055977215830

      Documentation

      Anti-α-Epithelial Cell Sodium Channel (20-42) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-α-Epithelial Cell Sodium Channel (20-42) Rabbit pAb Certificates of Analysis

      TitleLot Number
      324870

      References

      Reference overview
      Benos, D.J., and Stanton, B.A. 1999. J. Physiol. London 520, 631.
      Mano, I., and Driscoll, M. 1999. Bioessays 21, 568.
      Fyfe, G.K., et al. 1998. Semin. Nephrol. 18, 138.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision01-August-2007 RFH
      SynonymsAnti-α-ENaC
      ApplicationImmunoblotting (1 µg/ml)
      Immunoprecipitation (see comments)
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~97 kDa glycosylated α-ENaC protein.
      BackgroundEpithelial sodium channels are amiloride-sensitive members of the degenerin/epithelial sodium channel (Deg/ENaC) superfamily of ion channels. ENaC in the kidney, lung, and colon plays a role in trans-epithelial Na+ and fluid balance. ENaC also mediates aldosterone-dependent sodium reabsorption in the distal nephron of the kidney, thus regulating blood pressure. ENaC is believed to be regulated through association with the cystic fibrosis transmembrane conductance regulator (CFTR) chloride ion channel.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide [LMKGNKREEQGLGPEPAAPQQPT(C)] corresponding to amino acids 20-42 of human α-epithelial cell sodium channel (α-ENaC), conjugated to carrier protein
      IsotypeIgG
      Specieshuman, rat
      FormLiquid
      FormulationIn PBS, 1 mg/ml BSA.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsDoes not cross-react with β-ENaC or γ-ENaC. Also reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesBenos, D.J., and Stanton, B.A. 1999. J. Physiol. London 520, 631.
      Mano, I., and Driscoll, M. 1999. Bioessays 21, 568.
      Fyfe, G.K., et al. 1998. Semin. Nephrol. 18, 138.