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MABE656 Anti-HP1γ Antibody, clone 14D3.1

Anti-HP1γ, clone 14D3.1, Cat. No. MABE656, is a highly specific mouse monoclonal antibody that targets heterochromatin protein-1 gamma and has been tested in Immunocytochemistry and Western Blotting.

Anti-HP1γ Antibody, clone 14D3.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: CBX3 UniProt Number: Q13185
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MABE656
100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICC, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE656
      ReplacesMAB3450
      DescriptionAnti-HP1γ Antibody, clone 14D3.1
      Alternate Names
      • Chromobox protein homolog 3
      • HECH
      • Heterochromatin protein 1 homolog gamma
      • HP1 gamma
      • Modifier 2 protein
      Background InformationChromobox protein homolog 3 (UniProt Q13185; also known as HECH, Heterochromatin protein 1 homolog gamma, HP1 gamma, Modifier 2 protein) is encoded by the CBX3 gene (Gene ID 11335) in human. HP1 is a non-histone chromosomal protein that is present as three orthologs, HP1-alpha, HP1-beta, and HP1-gamma, in mammals. While HP1-alpha and HP1-beta have been unambiguously localized in heterochromatin, HP1-gamma has been found to localize in both euchromatin and heterochromatin (transiently) compartments of interphase nuclei, as well as in the pericentromeric chromatin and arms of mitotic chromosomes of 3T3 cells. HP1-gamma is phosphorylated by PIM1 during interphase and may be hyper-phosphorylated during mitosis. HPI-gamma is involved in transcriptional silencing in heterochromatin-like complexes. It recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. It may also contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). It is also reported to be involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Ref.: Minc E., et al. (2000). Cytogenet. Cell Genet. 90, 279-284.
      Lachner, M., et al. (2001). Nature 410, 116-120.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-HP1γ, clone 14D3.1, Cat. No. MABE656, is a highly specific mouse monoclonal antibody that targets heterochromatin protein-1 gamma and has been tested in Immunocytochemistry and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Application NotesImmunocytochemistry Analysis: A 1:200 dilution from a representative lot detected nuclear HP1γ immunoreactivity in 4% paraformaldehyde-fixed, 0.1 Triton X-100-permeabilized A431 and HeLa cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to an internal sequence within the N-terminal half of human HP1γ.
      Clone14D3.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Mouse, Non-Human Primate, and Rat based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CBX3
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~20 kDa observed. 20.68 kDa(Met1 removed) calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected HP1γ in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE656 04054839105555