05-233
Anti-Nitrotyrosine Antibody, clone 1A6
clone 1A6, Upstate®, from mouse
Synonym(s):
Anti-nitrotyrosine antibody
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
1A6, monoclonal
Species reactivity:
vertebrates
Application:
ICC, IHC, IP, WB
Citations:
160
biological source
mouse
Quality Segment
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
1A6, monoclonal
species reactivity
vertebrates
manufacturer/tradename
Upstate®
technique(s)
immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG2bκ
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... NOS1(4842)
General description
Nitrotyrosine has been identified as an indicator of cell damage and inflammation, as well as of the production of NO. It is believed that measuring the concentration of nitrotyrosine will serve as a marker for damage caused by NO in the cell. Previous studies have shown that nitrotyrosine has been found in inflammatory conditions such as atherosclerotic plaques and rheumatoid arthritis, among others
Predicted MW for nitrated-BSA: 66 kDa
Application
Anti-Nitrotyrosine Antibody, clone 1A6 detects level of Nitrotyrosine & has been published & validated for use in IC, IH, IP & WB.
Physical form
Format: Purified
Protein G Purified immunoglobulin in Protein G Purified immunoglobulin in 0.014M phosphate buffer, pH 7.6, 0.175M NaCl, 0.07% sodium azide, and 30% glycerol.
Analysis Note
Control
Immunohistochemistry: Human Alzheimer disease brain sections (Methacarn fixed), mouse liver
Immunoblotting: Nitrated-BSA
Indirect ELISA: nitrated-BSA
Indirect immunofluorescence: A549 cells metabolically labeled with 3-nitrotyrosine
Immunohistochemistry: Human Alzheimer disease brain sections (Methacarn fixed), mouse liver
Immunoblotting: Nitrated-BSA
Indirect ELISA: nitrated-BSA
Indirect immunofluorescence: A549 cells metabolically labeled with 3-nitrotyrosine
routinely evaluated on peroxynitrite-treated A431 cells; no reaction was noted with non-nitrated proteins
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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