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ABC1428 Anti-RecQ1

Anti-RecQ1, Cat. No. ABC1428, is a rabbit polyclonal antibody that detects ATP-dependent DNA helicase Q1 and has been tested for use in Immunofluorescence, Immunohistochemistry, and Western Blotting.

Anti-RecQ1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: RECQL, RECQ1, RECQL1 UniProt Number: P46063
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ABC1428
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIF, IHC, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberABC1428
      DescriptionAnti-RecQ1
      Alternate Names
      • ATP-dependent DNA helicase Q1
      • RecQ-like type 1
      • DNA-dependent ATPase Q1
      • RecQ protein-like 1
      Background InformationATP-dependent DNA helicase Q1 (UniProt: P46063; also known as EC:3.6.4.12, DNA helicase, RecQ-like type 1, RecQ1, DNA-dependent ATPase Q1, RecQ protein-like 1) is encoded by the RECQL (also known as RECQ1, RECQL1) gene (Gene ID: 5965) in human. RecQ helicases are a ubiquitous family of DNA unwinding enzymes involved in the maintenance of chromosome stability. They participate in various types of DNA repair, including mismatch repair, nucleotide excision repair and direct repair. Five members of the RecQ family have been reported in human cells: BLM, RECQ1, RECQ4, RECQ5, and WRN. RecQ1 displays high expression in heart, lung, skeletal muscle and kidney. It is also highly expressed in tumor cells and although brain exhibits low level of EecQ1, its expression is dramatically increased in human glioblastoma. Depletion of RecQ1 affects proliferation of glioblastoma cells and causes an increase in the level of DNA damage. RecQ1 depleted tumor cells are shown to be more sensitive to anti-tumor agents, such as hydroxyurea and temozolomide.
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing Tris-citrate-phosphate buffer (pH 7.8) with 0.09% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-RecQ1, Cat. No. ABC1428, is a rabbit polyclonal antibody that detects ATP-dependent DNA helicase Q1 and has been tested for use in Immunofluorescence, Immunohistochemistry, and Western Blotting.
      Key Applications
      • Immunofluorescence
      • Immunohistochemistry
      • Western Blotting
      Application NotesImmunofluorescence Analysis: A representative lot detected RecQ1 in T98G cells after treatment with anti-RECQ1 siRNA or control siRNAs (Mendoza-Maldonado, R., et. al. (2011). Mol Cancer. 10:83).

      Western Blotting Analysis: A representative lot detected RecQ1 in Western Blotting applications (Thangavel, S., et. al. (2015). J Cell Biol. 208(5):545-62).

      Immunohistochemistry Analysis: A representative lot detected RecQ1 in tissues are perilesional sections from multiform glioblastoma, colon carcinoma,lung cancer and thyroid cancer (Mendoza-Maldonado, R., et. al. (2011). Mol Cancer. 10:83).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal region of human ATP-dependent DNA helicase Q1 (RecQ1).
      EpitopeC-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects ATP-dependent DNA helicase Q1 (RecQ1) in human cells. It targets an epitope within 16 amino acids from the C-terminal end.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • RECQL
      • RECQ1
      • RECQL1
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~75 kDa observed; 73.46 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in IMR90 cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected RecQ1 in 10 µg of IMR90 human fetal lung cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABC1428 04054839328787