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ABS1676 Anti-VPS25

Anti-VPS25, Cat. No. ABS1676, is a rabbit polyclonal antibody that targets Vacuolar protein-sorting-associated protein 25 and has been tested for use in Immunofluorescence and Western Blotting.

Anti-VPS25 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: VPS25, DERP9, EAP20 UniProt Number: Q9BRG1
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ABS1676
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Mk, HIF, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberABS1676
      DescriptionAnti-VPS25
      Alternate Names
      • Vacuolar protein-sorting-associated protein 25
      • hVps25
      • Dermal papilla-derived protein 9
      • ELL-associated protein of 20 kDa
      • ESCRT-II complex subunit VPS25
      Background InformationVacuolar protein-sorting-associated protein 25 (UniProt: Q9BRG1; also known as hVps25, Dermal papilla-derived protein 9, ELL-associated protein of 20 kDa, ESCRT-II complex subunit VPS25) is encoded by the VPS25 (also known as DERP9, EAP20) gene (Gene ID: 84313) in human. VPS25 is a component of the ESCRT-II complex (endosomal sorting complex required for transport II) that is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. VPS25 is diffusely distributed throughout the cytoplasm and nucleoplasm, but exhibits a punctate distribution on co-expression with CHMP6. It is expressed at the mRNA level in kidney, liver, pancreas, and placenta. Lower levels of expression have been reported in heart, skeletal muscle, brain and lung.
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-VPS25, Cat. No. ABS1676, is a rabbit polyclonal antibody that targets Vacuolar protein-sorting-associated protein 25 and has been tested for use in Immunofluorescence and Western Blotting.
      Key Applications
      • Immunofluorescence
      • Western Blotting
      Application NotesImmunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected VPS25 in Cos-7 cells (Langelier, C., et. al. (2006). J Virol. 80(19):9465-80).

      Western Blotting Analysis: A 1:500 & 1:1,000 dilution from a representative lot detected VPS25 in lysate of transfected HEK293T cells (Sandrin, V., et. al. (2013). Retrovirology. 10:104; Langelier, C., et. al. (2006). J Virol. 80(19):9465-80).
      Biological Information
      ImmunogenGST-tagged full length human recombinant VPS25 protein.
      Epitopeunknown
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antibody specifically detects Vacuolar protein-sorting-associated protein 25 (VPS25) in human and monkey cells.
      IsotypeIgG
      Species Reactivity
      • Monkey
      • Human
      Species Reactivity NoteHuman, Monkey.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • VPS25
      • DERP9
      • EAP20
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~20 kDa observed; 20.75 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

      Western Blotting Analysis: A 1:2,000 dilution of this antibody detected VPS25 in 10 µg of HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABS1676 04054839201394