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05-1521 Anti-hnRNP A1 Antibody, clone 4B10

Use Anti-hnRNP A1 Antibody, clone 4B10 (mouse monoclonal antibody) validated in WB to detect hnRNP A1 also known as Helix-destabilizing protein, Single-str& RNA-binding protein, heterogeneous nuclear ribonucleoprotein A1.

Anti-hnRNP A1 Antibody, clone 4B10 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: RNA Metabolism & Binding Proteins Gene Symbol: HNRNPA1, HNRPA1, hnRNPA1 UniProt Number: P09651
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05-1521
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1521
      DescriptionAnti-hnRNP A1 Antibody, clone 4B10
      Alternate Names
      • Helix-destabilizing protein
      • Single-strand RNA-binding protein
      • heterogeneous nuclear ribonucleoprotein A1
      • heterogeneous nuclear ribonucleoprotein A1B protein
      • heterogeneous nuclear ribonucleoprotein B2 protein
      • heterogeneous nuclear ribonucleoprotein core protein A1
      • hnRNP core protein A1
      • nuclear ribonucleoprotein particle A1 protein
      • single-strand DNA-binding protein UP1
      Background InformationThe hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA) and associate with pre-mRNAs in the nucleus. These complexes are associated with pre-mRNA processing and other aspects of mRNA metabolism and transport. While all hnRNPs are present in the nucleus, data suggests they shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by hnRNP A1 has two repeats of quasi-RRM domains that bind to RNAs. This abundant core protein, along with other hnRNP proteins, is exported from the nucleus, probably bound to mRNA, and is immediately re-imported. The hnRNP A1 protein is involved in the packaging of pre-mRNA into hnRNP particles, transport of poly A+ mRNA from the nucleus to the cytoplasm, and may have a role in splice site selection.
      References
      Product Information
      FormatPurified
      Control
      • K562 cell lysate
      PresentationPurified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationUse Anti-hnRNP A1 Antibody, clone 4B10 (mouse monoclonal antibody) validated in WB to detect hnRNP A1 also known as Helix-destabilizing protein, Single-str& RNA-binding protein, heterogeneous nuclear ribonucleoprotein A1.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenFull length native protein partially purified human hnRNP A1.
      EpitopeUnknown
      Clone4B10
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes hnRNP A1.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It is one of the most abundant core proteins of hnRNP complexes and it is localized to the nucleoplasm. This protein, along with other hnRNP proteins, is exported from the nucleus, probably bound to mRNA, and is immediately re-imported. Its M9 domain acts as both a nuclear localization and nuclear export signal. The encoded protein is involved in the packaging of pre-mRNA into hnRNP particles, transport of poly A+ mRNA from the nucleus to the cytoplasm, and may modulate splice site selection. It is also thought have a primary role in the formation of specific myometrial protein species in parturition. Multiple alternatively spliced transcript variants have been found for this gene but only two transcripts are fully described. These variants have multiple alternative transcription initiation sites and multiple polyA sites. [provided by RefSeq].
      Gene Symbol
      • HNRNPA1
      • HNRPA1
      • hnRNPA1
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and may modulate splice site selection. May play a role in HCV RNA replication.

      SUBUNIT STRUCTURE: Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRNPA1, HNRNPA2B1, HNRPA3, HNRNPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRNPR, HNRNPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, TFIP11, THOC4, U2AF1, WDR57, XAB2 and ZCCHC8. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with SEPT6. Interacts with HCV NS5B and with the 5'-UTR and 3'-UTR of HCV RNA.

      SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles continuously between the nucleus and the cytoplasm along with mRNA. Component of ribonucleosomes. In the course of viral infection, colocalizes with HCV NS5B at speckles in the cytoplasm in a HCV-replication dependent manner.

      PTM: Arg-194, Arg-206 and Arg-225 are dimethylated, probably to asymmetric dimethylarginine.

      SEQUENCE SIMILARITIES: Contains 2 RRM (RNA recognition motif) domains.
      Molecular Weight ~ 38 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in K562 cell lysate.

      Western Blot Analysis: 0.1 µg/ml of this antibody detected HnRNP A1 on 10 µg of K562 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      05-1521 04053252514203

      Documentation

      Anti-hnRNP A1 Antibody, clone 4B10 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-hnRNP A1 Antibody, clone 4B10 Certificates of Analysis

      TitleLot Number
      Anti-hnRNP A1, clone 4B10 3012252
      Anti-hnRNP A1, clone 4B10 - 2199726 2199726
      Anti-hnRNP A1, clone 4B10 - 2251769 2251769
      Anti-hnRNP A1, clone 4B10 - 2561743 2561743
      Anti-hnRNP A1, clone 4B10 - 3190919 3190919
      Anti-hnRNP A1, clone 4B10 - 3543915 3543915
      Anti-hnRNP A1, clone 4B10 - 3734531 3734531
      Anti-hnRNP A1, clone 4B10 - 3832733 3832733
      Anti-hnRNP A1, clone 4B10 - 4086933 4086933
      Anti-hnRNP A1, clone 4B10 - 4123903 4123903
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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies