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344291 Freund’s Incomplete Adjuvant, Modified

Freund’s Incomplete Adjuvant, Modified Contains 85% Drakeol 5NF, 15% Arlacel A. Heat sterilized. Contains zero colony forming units.

Freund’s Incomplete Adjuvant, Modified MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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344291
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      Description
      OverviewContains 85% Drakeol 5NF, 15% Arlacel A (mannide monooleate emulsifier). Heat sterilized. Contains zero colony forming units/ml.
      Catalogue Number344291
      Brand Family Calbiochem®
      References
      ReferencesFreund, J. 1947. Ann. Rev. Microbiol. 1, 291.
      Product Information
      FormYellow oily liquid
      Quality LevelMQ100
      Applications
      ApplicationFreund’s Incomplete Adjuvant, Modified Contains 85% Drakeol 5NF, 15% Arlacel A. Heat sterilized. Contains zero colony forming units.
      Application Comments10 ml of Freund's Incomplete Adjuvant homogenized in the following amounts by weight:

      85% Drakeol 5 NF (light mineral oil)
      15% Arlacel A, mannide monooleate emulsifier.

      1. Take the vial of Friend's Incomplete Adjuvant and fold the aluminum seal back to permit free access to the stopper. Be careful not to break the aluminum ring that holds the stopper in place.
      2. Dissolve 50 mg of antigen in 5.0 ml of sterile PBS, pH 7.0-7.3. Maintain or create sterility if at all feasible.
      3. Draw the solution into a 10 ml syringe with a 1 inch 21 gauge needle.
      4. Insert needle into adjuvant vial and forcibly drive solution into the vial. Withdraw about half the mixture into syringe and force it into the adjuvant vial again.
      5. Repeat the mixing procedure in step 4 until a smooth, stable, milky, water-in-oil emulsion forms. Check if the emulsion is complete by expelling a drop from the syringe onto the water surface in a beaker. If the drop remains on the surface and does not disperse when the beaker is agitated slightly, the emulsion is adequate. A properly prepared emulsion is stable for days.
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +15°C to +30°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      344291-10MLCN 04055977214703

      Documentation

      Freund’s Incomplete Adjuvant, Modified SDS

      Title

      Safety Data Sheet (SDS) 

      Freund’s Incomplete Adjuvant, Modified Certificates of Analysis

      TitleLot Number
      344291

      References

      Reference overview
      Freund, J. 1947. Ann. Rev. Microbiol. 1, 291.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision26-June-2008 RFH
      DescriptionThe word "adjuvant" is derived from the Latin, adjuvare, meaning "to help." During immunization, an adjuvant is generally mixed with the antigen to enhance the rate and amount of antibody formed. The most popular complete adjuvant is that of Freund. It consists of killed and dried microbial cells suspended in an emulsifying oil. Adjuvant without the microbial cells is called "incomplete adjuvant." Complete adjuvant forms a stable water-in-oil emulsion with aqueous solution of antigen. When injected subcutaneously into animals, the emulsified antigen forms a depot from which antigen is delivered slowly and at sustained levels to the animal's antibody-synthesizing system. The induced antibody response is usually high and sustained. In guinea pigs, an additional benefit from adjuvant is the delayed appearance of hypersensitivity.
      FormYellow oily liquid
      SolubilityComposition
      10 ml of Freund's Incomplete Adjuvant homogenized in the following amounts by weight:
      85% Drakeol 5 NF (light mineral oil)
      15% Arlacel A, mannide monooleate emulsifier

      Sample Preparation
      1. Take the vial of Friend's Incomplete Adjuvant and fold the aluminum seal back to permit free access to the stopper. Be careful not to break the aluminum ring that holds the stopper in place.
      2. Dissolve 50 mg of antigen in 5 ml sterile PBS, pH 7.0-7.3. Maintain or create sterility if at all feasible.
      3. Draw the solution into a 10 ml syringe with a 1 inch 21 gauge needle.
      4. Insert needle into adjuvant vial and forcibly drive solution into the vial. Withdraw about half the mixture into syringe and force it into the adjuvant vial again.
      5. Repeat the mixing procedure in step 4 until a smooth, stable, milky, water-in-oil emulsion forms. Check if the emulsion is complete by expelling a drop from the syringe onto the water surface in a beaker. If the drop remains on the surface and does not disperse when the beaker is agitated slightly, the emulsion is adequate. A properly prepared emulsion is stable for days.
      Comments10 ml of Freund's Incomplete Adjuvant homogenized in the following amounts by weight:

      85% Drakeol 5 NF (light mineral oil)
      15% Arlacel A, mannide monooleate emulsifier.

      1. Take the vial of Friend's Incomplete Adjuvant and fold the aluminum seal back to permit free access to the stopper. Be careful not to break the aluminum ring that holds the stopper in place.
      2. Dissolve 50 mg of antigen in 5.0 ml of sterile PBS, pH 7.0-7.3. Maintain or create sterility if at all feasible.
      3. Draw the solution into a 10 ml syringe with a 1 inch 21 gauge needle.
      4. Insert needle into adjuvant vial and forcibly drive solution into the vial. Withdraw about half the mixture into syringe and force it into the adjuvant vial again.
      5. Repeat the mixing procedure in step 4 until a smooth, stable, milky, water-in-oil emulsion forms. Check if the emulsion is complete by expelling a drop from the syringe onto the water surface in a beaker. If the drop remains on the surface and does not disperse when the beaker is agitated slightly, the emulsion is adequate. A properly prepared emulsion is stable for days.
      Storage +15°C to +30°C
      Do Not Freeze Ok to freeze
      Toxicity Standard Handling
      ReferencesFreund, J. 1947. Ann. Rev. Microbiol. 1, 291.