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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

411483 Sigma-AldrichMouse Anti-Human IgG₃, Hinge Specific (HP6047) Biotin Conjugate

This Mouse Anti-Human IgG₃, Hinge Specific (HP6047) Biotin Conjugate is validated for use in ELISA for the detection of Human IgG₃, Hinge Specific.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
References
Data Sheet

Recommended Products

Overview

Replacement Information

Pricing & Availability

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Description
Catalogue Number	411483
Brand Family	Calbiochem®

References
References	Hamilton, R.G. 1990. Ann. Biol. Clin. 48, 473. Fasullo, F.J., et al. 1989. Clin. Chem. 35, 364. Hamilton, R.G. 1987. Clin. Chem. 33, 1707. Jefferis, R., et al. 1986. Immunol. Letters 10, 223.

Product Information
Form	Liquid
Formulation	In PBS, 1% BSA, pH 7.4.
Preservative	None
Quality Level	MQ100

Applications
Key Applications	Enzyme-Linked Immunosorbent Assay
Application Notes	ELISA (1:500-1:2000, see comments)
Application Comments	Biotinylated with hydroxysuccinimide ester biotin. This biotin-labeled antibody is an extremely versatile reagent because of the high binding affinity for avidin and avidin-labeled compounds. Variables associated with assay conditions will dictate the proper working dilution. Cross-reactivity by ELISA against human myeloma proteins: Human IgG₁: ≤0.1% Human IgG₂: ≤1% Human IgG₃: 100% Human IgG₄: ≤0.1% Human IgA: ≤0.1% Human IgE: ≤0.1% Human IgM: ≤0.1% Cross-reactivity by ELISA against chimeric antibodies: Human IgG₁: <0.001% Human IgG₂: <0.001% Human IgG₃: 100% Human IgG₄: <0.001% Human IgA: <0.001% Human IgE: <0.001% Human IgM: <0.001%

Biological Information
Immunogen	Human
Clone	HP6047
Host	Mouse
Isotype	IgG₃
Antibody Type	Monoclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Ambient Temperature Only
Toxicity	Standard Handling
Storage	-20°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
411483-200UGCN	04055977211245

Documentation

Mouse Anti-Human IgG₃, Hinge Specific (HP6047) Biotin Conjugate SDS

Title
Safety Data Sheet (SDS)

Mouse Anti-Human IgG₃, Hinge Specific (HP6047) Biotin Conjugate Certificates of Analysis

Title	Lot Number
411483	Enter Lot Number

References

Reference overview
Hamilton, R.G. 1990. Ann. Biol. Clin. 48, 473. Fasullo, F.J., et al. 1989. Clin. Chem. 35, 364. Hamilton, R.G. 1987. Clin. Chem. 33, 1707. Jefferis, R., et al. 1986. Immunol. Letters 10, 223.

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	26-February-2008 JSW
Application	ELISA (1:500-1:2000, see comments)
Description	Purified mouse monoclonal antibody conjugated to biotin. Recognizes human IgG₃ hinge region.
Host	Mouse
Immunogen species	Human
Clone	HP6047
Isotype	IgG₃
Form	Liquid
Formulation	In PBS, 1% BSA, pH 7.4.
Concentration Label	Please refer to vial label for lot-specific concentration
Preservative	None
Comments	Biotinylated with hydroxysuccinimide ester biotin. This biotin-labeled antibody is an extremely versatile reagent because of the high binding affinity for avidin and avidin-labeled compounds. Variables associated with assay conditions will dictate the proper working dilution. Cross-reactivity by ELISA against human myeloma proteins: Human IgG₁: ≤0.1% Human IgG₂: ≤1% Human IgG₃: 100% Human IgG₄: ≤0.1% Human IgA: ≤0.1% Human IgE: ≤0.1% Human IgM: ≤0.1% Cross-reactivity by ELISA against chimeric antibodies: Human IgG₁: <0.001% Human IgG₂: <0.001% Human IgG₃: 100% Human IgG₄: <0.001% Human IgA: <0.001% Human IgE: <0.001% Human IgM: <0.001%
Storage	Avoid freeze/thaw -20°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).
Toxicity	Standard Handling
References	Hamilton, R.G. 1990. Ann. Biol. Clin. 48, 473. Fasullo, F.J., et al. 1989. Clin. Chem. 35, 364. Hamilton, R.G. 1987. Clin. Chem. 33, 1707. Jefferis, R., et al. 1986. Immunol. Letters 10, 223.

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