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SHC001V

MISSION® pLKO.1-puro Empty Vector Control Transduction Particles

Contains no shRNA insert

Synonym(s):

MISSION®, MISSION® Control Transduction Particles

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About This Item

NACRES:
NA.51
UNSPSC Code:
41106609
Concentration:
≥1x106 VP/ml (via p24 assay)
Technique(s):
capture ELISA: 106 TU/mL using p24
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Quality Level

100

product line

MISSION®

concentration

≥1x106 VP/ml (via p24 assay)

technique(s)

capture ELISA: 106 TU/mL using p24

shipped in

dry ice

storage temp.

−70°C

General description

When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit sigma.com/shrna.

Application

To see more application data, protocols, vector maps visit sigma.com/shrna.

Legal Information

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
10112101MN
11041911MN
01021805MN

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Protocols

慢病毒转导实验方案

如何利用MISSION shRNA慢病毒颗粒进行慢病毒转导，以实现稳定的长期沉默和表型变化的详细操作步骤。

Lentiviral Transduction Protocol

Detailed procedure for how to perform a lentiviral transduction of MISSION shRNA lentiviral particles to achieve a stable long term silencing and phenotypic change.

Lentiviral Transduction Protocol

This protocol describes the use of MISSION TRCshRNA Lentiviral Particles and provides a system for long-term silencing andphenotypic observation.

Articles

Examination of Lentiviral Library Efficiency

Small interfering RNAs (siRNAs) are powerful tools for gene expression knockdown, widely used in molecular biology.

Efficacy of adjuvant chemotherapy for non-small cell lung cancer assessed by metastatic potential associated with ACTN4.
Miura N
Oncotarget (2016)
Deepak Parashar et al.
Cancer research, 80(24), 5554-5568 (2020-10-23)
Peritoneal spread is the primary mechanism of metastasis of ovarian cancer, and survival of ovarian cancer cells in the peritoneal cavity as nonadherent spheroids and their adherence to the mesothelium of distant organs lead to cancer progression, metastasis, and mortality.
Soft drug-resistant ovarian cancer cells invade via two distinct mechanisms utilizing myosin IIB
Aastha Kapoor
Nature, 099655-099655 (2017)
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