07-329-AF488
Anti-acetyl-Histone H4 (Lys16) Antibody, Alexa Fluor™ 488
from rabbit, ALEXA FLUOR™ 488
Synonym(s):
Histone H4, acetyl-Histone H4 (Lys16), AlexaFluor™ 488
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
ALEXA FLUOR™ 488
Clone:
polyclonal
Application:
ICC
Citations:
1
biological source
rabbit
Quality Segment
conjugate
ALEXA FLUOR™ 488
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, human
species reactivity (predicted by homology)
rat (based on 100% sequence homology)
technique(s)
immunocytochemistry: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
acetylation (Lys16)
Gene Information
human ... H4C1(8359)
mouse ... H4C1(326619)
General description
Histone H4 (UniProt P02309) is encoded by the HHF1 (ORF YBR0122; Gene ID 852294) and HHF2 (ORF N2752; Gene ID 855701) genes in yeast species. Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail, H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
~10 kDa observed
Immunogen
Epitope: H4K16ac
Ovalbumin-conjugated, synthetic peptide (…GAAcKRH …) in which AcK corresponds to residue 16 of yeast histone H4.
Application
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected acetyl-Histone H4 (Lys16) in A431 and NIH/3T3 cells.
The unconjugated antibody (Cat. No. 07-329) is shown to be suitable also for ChIP, ChIP-seq, Western blotting, multiplex assay, dot blot applications.
The unconjugated antibody (Cat. No. 07-329) is shown to be suitable also for ChIP, ChIP-seq, Western blotting, multiplex assay, dot blot applications.
This Anti-acetyl-Histone H4 (Lys16) Antibody, AlexaFluor 488 is validated for use in Immunocytochemistry for the detection of acetyl-Histone H4 (Lys16).
Biochem/physiol Actions
Broad species cross-reactivity is expected.
H4K16ac specificity of the unconjugated antibody (Cat. No. 07-329) has been confirmed using the Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and the Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).
Analysis Note
Evaluated by Immunocytochemistry in HeLa cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected acetyl-Histone H4 (Lys16) in HeLa cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected acetyl-Histone H4 (Lys16) in HeLa cells.
Other Notes
Concentration: Please refer to lot specific datasheet.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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