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EZCL-31K

Canine Leptin ELISA Kit

measures Leptin in 20 μL serum, plasma or cell culture supernatent

Synonym(s):

Canine Leptin Kit, Leptin ELISA

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84
Input:
sample type serum
sample type plasma (K2 EDTA)
Species reactivity:
canine
Storage temp.:
2-8°C
Shipped in:
wet ice
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Product Name

Canine Leptin ELISA,

species reactivity

canine

Quality Segment

200

packaging

kit of 1 × 96 wells

parameter

20 μL sample volume (4hr assay)

technique(s)

ELISA: suitable

input

sample type serum
sample type plasma (K2 EDTA)

application(s)

research use

assay range

inter-assay cv: 6-7%
intra-assay cv: 2-6%
sensitivity: 0.21 ng/mL
standard curve range: 0.78-50  ng/mL
accuracy: 100-106%

detection method

colorimetric (450nm/590nm)

shipped in

wet ice

storage temp.

2-8°C

General description

Leptin, a 16 kD protein and a product of the obesity (ob) gene, is synthesized and secreted predominantly by adipose tissue and is, therefore, considered an adipokine. With circulating levels directly proportional to the total amount of body fat, leptin plays an important role in whole body metabolism, regulating food intake and energy balance, as well as assisting in the control of homeostasis, reproductive function, the immune system and numerous other body functions. Binding to receptors of the hypothalamus, leptin signals food satiety and reduces appetite, although evidence suggests that, in general, obese people may have unusually high circulating levels of leptin. This may indicate a resistance to leptin similar to the insensitivity to insulin associated with type 2 diabetes. Consequently, the study of leptin is essential to understanding the relationship among appetite, energy balance and obesity.

Application

This Canine Leptin ELISA is used to measure & quantify Leptin levels in Metabolism & Endocrine & Neuroscience research.

This Is a 4-hour room temperature assay using 20 μL sample size

Research Category: Metabolism

Research Sub-Category: Obesity, Metabolic Disorders

Biochem/physiol Actions

Used to detect/quantify: Leptin

Packaging

96-well strip plate

Other Notes

Contact Technical Support.

Disclaimer

For research use only. Not for use in diagnostic procedures.

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - ED ENV 1 - Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

target_organs

Respiratory system

Storage Class

10 - Combustible liquids

wgk

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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