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L3540

Lowry Reagent

Synonym(s):

Copper(II) tartrate Mix

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About This Item

NACRES:
NA.32
UNSPSC Code:
12352200
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Quality Level

100

General description

The Lowry method has been for decades the procedure of choice for quantitation of soluble proteins due to its sensitivity, simplicity, and precision. Peterson′s modification of the micro Lowry method is simple, rapid, objective and more generally applicable. In this procedure sodium dodecyl sulfate (SDS) is mixed with Lowry reagent to enable the dissolution of relatively insoluble lipoproteins.

Application

Lowry Reagent has been used to quantify the protein content in extracellular polymeric substance (EPS). It has also been used to quantify total protein and mitochondrial protein in Drosophila melanogaster.
For protein determination. Utilizes SDS to facilitate dissolution of relatively insoluble lipoporoteins.

Preparation Note

Reconstitute to 40 mL with deionized water.

Other Notes

Dry powder mixture containing Lowry reagent with sodium dodecyl sulfate.

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Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Flam. Sol. 2 - Skin Corr. 1B

Storage Class

4.1B - Flammable solid hazardous materials

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

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Certificates of Analysis (COA)

Lot/Batch Number
0000547499
0000547499
0000491218
0000501864
0000501864

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Protocols

Protein Determination by the Warburg-Christian Method

To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.

Protein Determination Modified Lowry Method

To standardize a procedure for the determination of protein by modified Lowry.

Related Content

Protein Quantitation

Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.

Tools for Protein Quantitation

Products for traditional and alternative protein quantitation techniques available, including BCA, Bradford, Lowry, and more.

蛋白定量

蛋白定量方法、试剂和免疫测定技术可准确测定各类样品中的蛋白浓度。

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Kim HS and Park HD
PLoS ONE, 8(9), e76106-e76106 (2013)
Environmental and genetic perturbations reveal different networks of metabolic regulation
Greenberg A, et al.
Molecular Systems Biology, 7(1), 563-563 (2011)
A simplification of the protein assay method of Lowry et al. which is more generally applicable
Peterson GL
Analytical Biochemistry, 83(2), 346-356 (1977)
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