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About This Item
NACRES:
NB.22
UNSPSC Code:
41121702
Capacity:
0.2 mL
Material:
flat cap, colorless cap, colorless tube, conical bottom, polypropylene , polypropylene cap, polypropylene tube
Sterility:
non-sterile
Packaging:
pack of 250 ea, case of 4 packs
Feature:
RNase and DNase free, autoclavable at 121 °C, graduations: no, Push fit
material
flat cap, colorless cap, colorless tube, conical bottom, polypropylene , polypropylene cap, polypropylene tube
description
Autoclavable
sterility
non-sterile
feature
RNase and DNase free, autoclavable at 121 °C, graduations: no, Push fit
packaging
pack of 250 ea, case of 4 packs
technique(s)
PCR: suitable
capacity
0.2 mL
color
clear
suitability
suitable for (PCR applications)
General description
Thin-walled for efficient thermal transfer and shorter cycle times; fits all leading thermal cyclers including PerkinElmer, Biometra, MJ Research, Techne, Grant and Strategene (0.65 mL only). Certified DNase- and RNase-free. All tolerate organic solvent reactions and temperatures from -4 to 121 °C.
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Protocols
使用抗体介导热启动聚合酶的实验方案。一种具有短活化阶段的方法（<1min), and results in higher yields and more specificity over standard PCR methods.
使用逆转录酶和dNTPs进行逆转录（RT）使用总RNA或基因特异性方法实现仅目标RNA被转化为cDNA。
逆转录是一种旨在测量基因mrna浓度的基因表达分析。 这种分析存在多个挑战，例如不同转录本之间的半衰期差异、时间模式和缺乏相关蛋白。