产品名称
BL21(DE3)化学感受态细胞, for protein expression
biological source
Escherichia coli
grade
Molecular Biology
growth mode
adherent or suspension
morphology
rod shaped
technique(s)
microbiological culture: suitable
cell transformation
competent cell type: chemically competent
transformation efficiency: ≥1 x 107 cfu/μg
shipped in
dry ice
storage temp.
−70°C
General description
BL21(DE3)化学感受态菌株含有与IPTG诱导型启动子连接的噬菌体T7 RNA聚合酶基因,可用于含有T7启动子的任何表达质粒。
基因型
F – ompT hsdSB (rB- mB-) gal dcm (DE3)
基因型
F – ompT hsdSB (rB- mB-) gal dcm (DE3)
Application
该 大肠杆菌 BL21(DE3)菌株已用于:以pET19b作为表达载体,表达tau 2N4R同种型(aa1–441)和截短的tau(aa1–421)。BL21(DE3)化学感受态细胞已被用于protegrin-1 (PG1) 肽的转化和表达。 在 pHis2载体中表达6His-TEVsite-Hs ARF1 (17-181)-Q71L。
Features and Benefits
- 转化效率> 1×107 CFU /µg
- 实惠的价格
- 可为日常蛋白表达工作提供卓越价值。
Other Notes
- BL21(DE3)化学感受态细胞
- pUC19转化对照DNA
- 表达恢复培养基
存储类别
10 - Combustible liquids
wgk
WGK 2
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