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Merck

CMC0014

BL21(DE3)化学感受态细胞

Escherichia coli, rod shaped

别名:

感受态大肠杆菌细胞

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关于此项目

UNSPSC Code:
41106202
NACRES:
NA.85
Biological source:
Escherichia coli
Growth mode:
adherent or suspension
Morphology:
rod shaped
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产品名称

BL21(DE3)化学感受态细胞, for protein expression

biological source

Escherichia coli

grade

Molecular Biology

growth mode

adherent or suspension

morphology

rod shaped

technique(s)

microbiological culture: suitable

cell transformation

competent cell type: chemically competent
transformation efficiency: ≥1 x 107 cfu/μg

shipped in

dry ice

storage temp.

−70°C

General description

BL21(DE3)化学感受态菌株含有与IPTG诱导型启动子连接的噬菌体T7 RNA聚合酶基因,可用于含有T7启动子的任何表达质粒。

基因型

F – ompT hsdSB (rB- mB-) gal dcm (DE3)

Application

大肠杆菌 BL21(DE3)菌株已用于:以pET19b作为表达载体,表达tau 2N4R同种型(aa1–441)和截短的tau(aa1–421)。
  • BL21(DE3)化学感受态细胞已被用于protegrin-1 (PG1) 肽的转化和表达。
  • 在 pHis2载体中表达6His-TEVsite-Hs ARF1 (17-181)-Q71L。
  • Features and Benefits

    • 转化效率> 1×107 CFU /µg
    • 实惠的价格
    • 可为日常蛋白表达工作提供卓越价值。

    Other Notes

    • BL21(DE3)化学感受态细胞
    • pUC19转化对照DNA
    • 表达恢复培养基



    存储类别

    10 - Combustible liquids

    wgk

    WGK 2



    历史批次信息供参考:

    分析证书(COA)

    Lot/Batch Number

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