Quality Level
form
liquid
concentration
1 unit/μL
technique(s)
RT-PCR: suitable
color
colorless
shipped in
wet ice
storage temp.
−20°C
General description
DNase I可将双链和单链DNA酶解成寡核苷酸和单核苷酸。采用提供的反应缓冲液，在室温下消化 15 分钟，可去除 RNA 制剂中的 DNA。然后随同终止液加热，使DNase I失活。加热还会使RNA中的发夹变性，可直接用于逆转录。
当前没有任何 RNA 分离程序可以 100% 去除 DNA。 许多商业DNase I 制剂受到了残留RNase污染。 这种RNase污染会在逆转录以前破坏或分解有价值的RNA样品。实验室比较表明，Sigma′的扩增级 DNase I 的RNase活性低于几家领先的分子生物学产品供应商产品。
Application
- 在 RNA 的分离和纯化过程中消化 DNA。纯化的 RNA 可用于通过RNA 逆转录酶合成 cDNA。
- 水解细胞外基质 (ECM) 成分并增强光敏剂在生物膜中的渗透以确定抗菌光动力疗法 (aPDT) 对白色念珠菌生物膜的功效
- 从牛血样中提取的总 RNA 中去除污染 DNA
Features and Benefits
- 适用于去除 RNA 中的 DNA
- 超低RNase活性
- 经过优化的10×反应缓冲液和终止液，可使DNase I完全失活
Other Notes
Legal Information
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
实验方案
将RNA逆转录成DNA的方法。使用含有逆转录酶、dNTP、引物、RNase抑制剂和缓冲液的预混试剂。快速合成cDNA。
SeqPlex RNA扩增试剂盒可在进入常用深度测序平台工作流程之前为总RNA或分离后的mRNA提供一种扩增方法，
Method for reverse transcription of RNA into DNA. Uses a premixed reagent that contains reverse transcriptase, dNTPs, primers, RNase inhibitor and buffer. Fast generation of cDNA.
商品
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SeqPlex™-I WTA kit amplifies RNA for NGS, enabling genomic studies from limited samples.
Use of MULTI-seq lipid-modified oligos, protocol, and troubleshooting guide for PCR Assays and Sequencing applications.
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