D2532
Denhardt溶液 50x
for Northern and Southern blotting, solution
别名:
blocking reagent, blocking solution, denhardts, hybridization buffer, hybridization solution, northern blocking solution, pre-hybridization, southern blocking solution
登录 查看组织和合同定价。
选择尺寸
变更视图
关于此项目
NACRES:
NA.25
UNSPSC Code:
12161700
Quality Level
form
solution
technique(s)
Southern blotting: suitable
foreign activity
DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected
storage temp.
−20°C
General description
Denhardt溶液是一种封闭剂，常规用于减少检测试剂与Northern和Southern印迹膜的非特异性结合。 这种50x浓缩液可以稀释到任何浓度，用于多种预杂交和杂交缓冲液。
Preparation Note
Denhardt溶液是1%牛血清白蛋白（BSA）、1%Ficoll和1%聚乙烯吡咯烷酮（PVP）的水溶液。
存储类别
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
实验方案
Ficoll 400®是由蔗糖和环氧氯丙烷共聚而成的一种高度分支化的聚合物。Ficoll 400®是完全非离子性的。由于其含有丰富的羟基，Ficoll 400®具有很强的亲水性和水溶性。
Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
Galanin receptor 3--a potential target for acute pancreatitis therapy.
Barreto SG
Neurogastroenterology and Motility, 23(3), 23(3)-23(3) (2011)
J Stokes et al.
Investigative ophthalmology & visual science, 41(7), 1629-1638 (2000-06-14)
The administration of glucocorticoids as topical or systemic medications may lead to the development of ocular hypertension through the induction of morphologic and biochemical changes in the trabecular meshwork leading to a reduction in the facility of aqueous outflow. Glucocorticoids
E J Mackie et al.
Journal of cell science, 103 ( Pt 3), 765-771 (1992-11-01)
The extracellular matrix glycoprotein, tenascin, is associated in vivo with mesenchyme undergoing osteogenesis and chondrogenesis, but is absent from mature bone and cartilage matrix. The expression of tenascin by osteoblastic cells in vitro has been investigated by immunoblotting and immunocytochemistry.