D8418
二甲基亚砜
Molecular Biology
别名:
分子生物学级DMSO, 甲基亚砜, 甲基硫酰基甲烷, DMSO
选择尺寸
关于此项目
grade
Molecular Biology
Quality Level
vapor density
2.7 (vs air)
vapor pressure
0.42 mmHg ( 20 °C)
product line
BioReagent
assay
≥99.9%
form
liquid
autoignition temp.
573 °F
shelf life
Recommended retest period - 2 years
expl. lim.
42 %, 63 °F
technique(s)
DNA sequencing: suitable, PCR: suitable, transfection: suitable
impurities
≤0.001 meq/g Titratable acid, ≤0.1% water (Karl Fischer)
color
colorless
refractive index
n20/D 1.479 (lit.)
bp
189 °C (lit.)
mp
16-19 °C (lit.)
solubility
H2O: miscible (completely)
density
1.10 g/mL (lit.)
absorption
passes test
suitability
suitable for molecular biology
foreign activity
DNase and RNase, none detected
storage temp.
room temp
SMILES string
CS(C)=O
InChI
1S/C2H6OS/c1-4(2)3/h1-2H3
InChI key
IAZDPXIOMUYVGZ-UHFFFAOYSA-N
General description
- 适于分子应用
- 无核酸酶DMSO
- 不含DNase、RNase、蛋白酶和磷酸酶
- 分子生物学级DMSO
二甲基亚砜（DMSO）是一种对有机和无机化学品具有出色溶剂性质的高极性有机试剂。
该产品为分子生物学级，适用于分子生物学应用，对其中核酸酶和蛋白酶的存在也进行了分析。
Application
- 聚合酶链式反应 (PCR)
- cDNA文库扩增
- DNA测序
- poly（A）+ RNA筛选的上柱缓冲液
- 感受态大肠杆菌转化缓冲液
- 转染方案。
二甲基亚砜已用于-
- 用作芯片点样应用中的寡核苷酸溶剂[1]
- 用作miRNA芯片分析和实时荧光定量PCR (qRT-PCR)中的溶剂
- 在MTT(3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四唑溴化物）测定的细胞裂解液
- 制备MMP（线粒体膜电位）细胞荧光评估过程所用的储备溶液
- 用作储存人类和动物细胞系和噬菌体λ的冷冻保护剂。
Features and Benefits
Other Notes
Disclaimer
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存储类别
10 - Combustible liquids
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
实验方案
酵母因能够快速生长并具有分散的细胞而被认为是用于真核生物研究的模范系统。
Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells.
Learn how to cultivate similar-sized iPSC-derived colon organoids using Millicell® Microwell plates and perform a forskolin-induced swelling assay.
商品
转化是将外源DNA引入细胞，引起遗传变异或基因修饰的过程。1928年，Griffith首次报道了利用肺炎链球菌进行的转化。Avery等人于1944年证明了DNA转化的原理。
Transformation introduces exogenous DNA into cells, a fundamental genetic modification process demonstrated in Streptococcus pneumoniae.
SeqPlex™-I WTA kit amplifies RNA for NGS, enabling genomic studies from limited samples.
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