Quality Level
analyte chemical class(es)
sugars (glucose)
technique(s)
photometry: suitable
impurities
≤0.005% Creatine phosphokinase, glutathione reductase, myokinase, NADH-oxidase,NADPH oxidase, phosphogluconic dehydrogenase, phosphoglucose isomerase, lactic dehydrogenase, <0.01% ATP-ase, myokinase
application(s)
food and beverages
general analytical
storage temp.
2-8°C
Application
加入包装所示体积（20mL 或 50mL）的去离子水。
通过己糖激酶-葡萄糖-6-磷酸的酶法对食物和其他物质中的葡萄糖进行定量测定。
Biochem/physiol Actions
与以下底物发生交叉反应可导致活性 < 0.05%：果糖、葡聚糖、景天庚糖、阿拉伯糖、半乳糖、木糖、海藻糖、甘露糖、山梨醇、+/-岩藻糖或核糖。
存储类别
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
实验方案
改良Boehenger法酶促检测α-葡萄糖苷酶(EC 3.2.1.20)
本操作程序可用于使用淀粉作为底物测定淀粉葡萄糖苷酶的活性。
该实验方案使用分光光度法测定纤维素酶活性。黑曲霉纤维素酶催化内-1,4-β-D-糖苷键的水解。
相关内容
Instructions
Daniel S Straus
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Mor Tsamir-Rimon et al.
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Michele Bertazzini et al.
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