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关于此项目
线性分子式:
H2O
化学文摘社编号:
分子量:
18.02
NACRES:
NA.25
PubChem Substance ID:
UNSPSC Code:
12191602
EC Number:
231-791-2
MDL number:
Beilstein/REAXYS Number:
2050024
Technique(s):
PCR: suitable
Bp:
100 °C (lit.)
Vapor pressure:
3 mmHg
产品名称
水, PCR Reagent
vapor density
<1 (vs air)
Quality Level
vapor pressure
3 mmHg
sterility
sterile-filtered
form
liquid
packaging
vial of 1.5 mL
technique(s)
PCR: suitable
refractive index
n20/D 1.34 (lit.)
pH
5-7
bp
100 °C (lit.)
mp
0 °C (lit.)
density
1.000 g/mL at 3.98 °C (lit.)
foreign activity
DNase, none detected, RNase, none detected
SMILES string
O
InChI
1S/H2O/h1H2
InChI key
XLYOFNOQVPJJNP-UHFFFAOYSA-N
General description
对 PCR 级水进行无菌过滤。它不含核酸外切酶(dna 酶、rna 酶)和核酸内切酶(切口酶),也不含核酸污染。
Application
适用于聚合酶链式反应 (PCR)
水已用于:
- 作为反应混合物的组分和微流控RT-qPCR的稀释剂
- 作为反应混合物的组分,以扩增真菌(杂色云芝)DNA的产物
- 作为稀释剂和反应混合物的组分,以扩增cDNA
- 水已用于在聚合酶链式反应 (PCR) 中补充样品的最终体积
Other Notes
便于将水产品规格与 水规格表进行比较。
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存储类别
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
实验方案
使用抗体介导热启动聚合酶的实验方案。一种具有短活化阶段的方法(<1min), and results in higher yields and more specificity over standard PCR methods.
使用逆转录酶和dNTPs进行逆转录(RT)使用总RNA或基因特异性方法实现仅目标RNA被转化为cDNA。
逆转录是一种旨在测量基因mrna浓度的基因表达分析。 这种分析存在多个挑战,例如不同转录本之间的半衰期差异、时间模式和缺乏相关蛋白。
Multiplex PCR for rapid detection of genes encoding acquired metallo-beta-lactamases.
Matthew J Ellington et al.
The Journal of antimicrobial chemotherapy, 59(2), 321-322 (2006-12-23)
Incidence and survival of non-O157 verocytotoxigenic Escherichia coli in soil
Bolton DJ, et al.
Journal of Applied Microbiology, 111(2), 484-490 (2011)
N Wellinghausen et al.
Applied and environmental microbiology, 67(9), 3985-3993 (2001-08-30)
Contamination of hospital water systems with legionellae is a well-known cause of nosocomial legionellosis. We describe a new real-time LightCycler PCR assay for quantitative determination of legionellae in potable water samples. Primers that amplify both a 386-bp fragment of the