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ASK23 Downstream Effector Caspase Sampler Kit

Downstream Effector Caspase Sampler Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
ASK23
  
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Přehled

Replacement Information
Description
OverviewContains 20 µg of each of the following antibodies: Anti-Caspase-3 (Cat. Nos. AM34 and AM39), Anti-Caspase-6 (Cat. Nos. PC365 and PC416), and Anti-Caspase-7 (Cat. Nos. AM45 and PC334). Please see individual product descriptions for further information.
Catalogue NumberASK23
Brand Family Calbiochem®
References
ReferencesCryns, V., and Yuan, J., 1998. Genes Dev. 12, 1551.
Wolf, B., and Green, D., 1999. J. Biol. Chem. 274, 20049.
Ashkenazi, A., and Dixit, V., 1998. Science 281, 1305.
Cohen, G., 1997. Biochem. J. 326, 1.
Nicholson, S., and Thornberry, N., 1997. Trends Biochem. Sci. 22, 299.
Product Information
ProductIsotypeCloneSpecies ReactivityApplicationCat. No.
Caspase-3 (Ab-2) Monoclonal AntibodyIgG₁10C1.C9HumanWB, not FS, IF, IP or PS AM34
Caspase-3 (Ab-3) Monoclonal AntibodyIgG2aAM1.4.1-1B HumanWB, not FS, IP or PS AM39
Caspase-6 (Ab-1) Polyclonal AntibodyIgGHuman, MouseWB PC365
Caspase-6 (Ab-2) Polyclonal AntibodyIgGHuman, Mouse, RatWB PC416
Caspase-7 (Ab-1) Polyclonal AntibodyIgGHumanWB PC334
Caspase-7 (Ab-2) Monoclonal AntibodyIgG₁I-10Human, not Mouse or RatWB AM45
FormLiquid
Positive controlHeLa or MCF7 cells induced for apoptosis
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (see characteristics below)
Frozen Sections (see characteristics below)
Immunofluorescence (see characteristics below)
Immunoprecipitation (see characteristics below)
Paraffin Sections (see characteristics below)
Application CommentsSuggested starting concentrations are provided on individual data sheets. Both primary and secondary antibodies should be titrated for optimal results in individual systems.
Biological Information
IsotypeIgG
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
ASK23 0

Documentation

References

Přehled odkazů
Cryns, V., and Yuan, J., 1998. Genes Dev. 12, 1551.
Wolf, B., and Green, D., 1999. J. Biol. Chem. 274, 20049.
Ashkenazi, A., and Dixit, V., 1998. Science 281, 1305.
Cohen, G., 1997. Biochem. J. 326, 1.
Nicholson, S., and Thornberry, N., 1997. Trends Biochem. Sci. 22, 299.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision27-February-2008 RFH
ApplicationImmunoblotting (see characteristics below)
Frozen Sections (see characteristics below)
Immunofluorescence (see characteristics below)
Immunoprecipitation (see characteristics below)
Paraffin Sections (see characteristics below)
DescriptionSet of 6 mouse monoclonal antibodies specific for caspase-3, caspase-6, or caspase-7.
BackgroundThe caspase family of proteins plays a central role in the apoptotic response. Normally existing in a latent, zymogen form, following receipt of an apoptotic signal they become activated and proceed to cleave and dismantle the structural, regulatory and genetic information in the cell either directly or indirectly. There are currently over a dozen caspase proteins that have been described in multiple species and many of these proteins exist as isoform variants. The spectrum of caspases can be broadly divided into three families based either on structural motifs held in common or on the basis of activity. These families are the CARD containing caspases, the DED containing caspases and the downstream effector caspases. The downstream effector caspases are those responsible for direct cleavage of cellular substrates and most likely responsible for the "death-blow" during apoptosis. This group contains caspases 3, 6, and 7.
IsotypeIgG
Positive controlHeLa or MCF7 cells induced for apoptosis
FormLiquid
CommentsSuggested starting concentrations are provided on individual data sheets. Both primary and secondary antibodies should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesCryns, V., and Yuan, J., 1998. Genes Dev. 12, 1551.
Wolf, B., and Green, D., 1999. J. Biol. Chem. 274, 20049.
Ashkenazi, A., and Dixit, V., 1998. Science 281, 1305.
Cohen, G., 1997. Biochem. J. 326, 1.
Nicholson, S., and Thornberry, N., 1997. Trends Biochem. Sci. 22, 299.