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PC522 Anti-Adenine Nucleotide Translocase-1 (Ab-1) (2-11) Rabbit pAb

PC522
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      H Rb Polyclonal Antibody
      Description
      OverviewRecognizes the ~35 kDa ANT-1 protein in doxorubicin-treated MCF-7 cells or breast carcinoma tissue.
    • Antibody Target Gene Symbol: ANK3
    • Target Synonym: 2900054D09RIK, AI314020, AnkG, Ankyrin-3, ANKYRIN-G, FLJ45464, MGC14049
    • Entrez Gene Name: ankyrin 3, node of Ranvier (ankyrin G)
    • Hu Entrez ID: 288 (Related Antibodies: NB20, AP1034, PC523, AP1057)
    • Mu Entrez ID: 11735
    • Rat Entrez ID: 361833
    • Catalogue NumberPC522
      Brand Family Calbiochem®
      SynonymsAnti-ANT-1
      Application Data
      Detection of human ANT-1 by staining paraffin sections. Sample: Breast carcinoma. Primary antibody: Anti-Adenine Nucleotide Translocase-1 (Ab-1) (2-11) Rabbit pAb (Cat. No. PC522) (1 µg/ml). Detection: DAB with methyl green counterstain.
      References
      ReferencesBauer, M.K.A., et al. 1999. J. Cell Biol. 147, 1493.
      Marzo, I, et al. 1998. Science 281, 2027.
      Marzo, I, et al. 1998. J. Exp. Med. 187, 1261.
      Zoratti, M. and Szabo, I. 1995. Biochim. Biophys. Acta 1231, 139.
      Klingenberg, M. 1993. J. Bioenerg. Biomembr. 25, 447.
      Riccio, P., et al. 1975. FEBS Lett. 56, 133.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 50% glycerol.
      Positive controldoxorubicin-treated MCF7 cells, or breast carcinoma tissue
      PreservativeNone
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunofluorescence
      Paraffin Sections
      Application NotesImmunoblotting (2.5 µg/ml, chemiluminescence)
      Immunofluorescence (1 µg/ml)
      Paraffin Sections (1 µg/ml, heat pre-treatment required)
      Application CommentsMCF7 cells treated with doxorubicin at 0.2 µg/ml for 24 h can be used as positive control for immunoblotting. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids 2-11 of human ANT-1
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Adenine Nucleotide Translocase-1 (Ab-1) (2-11) Rabbit pAb MSDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Adenine Nucleotide Translocase-1 (Ab-1) (2-11) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC522

      References

      Reference overview
      Bauer, M.K.A., et al. 1999. J. Cell Biol. 147, 1493.
      Marzo, I, et al. 1998. Science 281, 2027.
      Marzo, I, et al. 1998. J. Exp. Med. 187, 1261.
      Zoratti, M. and Szabo, I. 1995. Biochim. Biophys. Acta 1231, 139.
      Klingenberg, M. 1993. J. Bioenerg. Biomembr. 25, 447.
      Riccio, P., et al. 1975. FEBS Lett. 56, 133.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision29-June-2007 RFH
      SynonymsAnti-ANT-1
      ApplicationImmunoblotting (2.5 µg/ml, chemiluminescence)
      Immunofluorescence (1 µg/ml)
      Paraffin Sections (1 µg/ml, heat pre-treatment required)
      Application Data
      Detection of human ANT-1 by staining paraffin sections. Sample: Breast carcinoma. Primary antibody: Anti-Adenine Nucleotide Translocase-1 (Ab-1) (2-11) Rabbit pAb (Cat. No. PC522) (1 µg/ml). Detection: DAB with methyl green counterstain.
      DescriptionPurified rabbit polyclonal antibody. Recognizes the ~35 kDa ANT-1 protein.
      BackgroundANT-1 (adenine nucleotide translocase-1) (~35 kDa) is a key component of the mitochondrial permeability transition pore (PT pore) complex and is also one of the most abundant proteins in the mitochondrion making up approximately 10% of all mitochondrial proteins. Originally described as an ADP/ATP exchange factor, ANT-1 has also been shown to be required for bax mediated apoptosis. This is effected by binding of bax to residues 105-156 of ANT-1, presumably resulting in maintaining the PT pore in an open conformation. Interaction with bax is not required for ANT-1 pro-apoptotic activity as overexpression of the ANT-1 protein also leads to the induction of apoptosis.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide corresponding to amino acids 2-11 of human ANT-1
      IsotypeIgG
      Specieshuman
      Positive controldoxorubicin-treated MCF7 cells, or breast carcinoma tissue
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 50% glycerol.
      Concentration Label Please refer to vial label for lot-specific concentration
      PreservativeNone
      CommentsMCF7 cells treated with doxorubicin at 0.2 µg/ml for 24 h can be used as positive control for immunoblotting. Antibody should be titrated for optimal results in individual systems.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesBauer, M.K.A., et al. 1999. J. Cell Biol. 147, 1493.
      Marzo, I, et al. 1998. Science 281, 2027.
      Marzo, I, et al. 1998. J. Exp. Med. 187, 1261.
      Zoratti, M. and Szabo, I. 1995. Biochim. Biophys. Acta 1231, 139.
      Klingenberg, M. 1993. J. Bioenerg. Biomembr. 25, 447.
      Riccio, P., et al. 1975. FEBS Lett. 56, 133.