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ST1589 Anti-PKM2 Mouse mAb (5D2-3B3)

ST1589
  
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      Overview

      Replacement Information

      Key Spec Table

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      Description
      Overview

      This product has been discontinued.





      Recognizes the ~55 kDa PKM2 protein in HeLa cells.
      Catalogue NumberST1589
      Brand Family Calbiochem®
      SynonymsAnti-Pyruvate Kinase, Muscle
      Application Data
      Detection of human PKM2 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-PKM2 Mouse mAb (5D2-3B3) (Cat. No. ST1589) (1-5 µg/ml). Detection: chemiluminescence.
      References
      Product Information
      FormLiquid
      FormulationUndiluted ascites
      Positive controlHeLa cells
      PreservativeNone
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1-5 µg/ml)
      Application CommentsVariables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenPKM2
      Clone5D2-3B3
      HostMouse
      IsotypeIgM
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision09-November-2010 RFH
      SynonymsAnti-Pyruvate Kinase, Muscle
      ApplicationImmunoblotting (1-5 µg/ml)
      Application Data
      Detection of human PKM2 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-PKM2 Mouse mAb (5D2-3B3) (Cat. No. ST1589) (1-5 µg/ml). Detection: chemiluminescence.
      DescriptionMouse monoclonal antibody supplied as undiluted ascites. Recognizes the ~55 kDa PKM2 protein.
      BackgroundPKM2 is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis.
      HostMouse
      ImmunogenPKM2
      Clone5D2-3B3
      IsotypeIgM
      Specieshuman
      Positive controlHeLa cells
      FormLiquid
      FormulationUndiluted ascites
      PreservativeNone
      CommentsVariables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review