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IM56 Anti-TIMP-2 (Ab-2) Mouse mAb (67-4H11)

IM56
  
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Overview

Replacement Information

Key Spec Table

Host
M
Description
Overview

This product has been discontinued.





Recognizes the ~21-28 kDa TIMP-2 protein.
Catalogue NumberIM56
Brand Family Calbiochem®
SynonymsAnti-Tissue Inhibitor of Metalloproteinase-2
Application Data
Detection of hamster TIMP-2 by immunoblotting. Sample: Expressed in CHO cells. Primary antibody: Anti-TIMP-2 (Ab-2) Mouse mAb (67-4H11) (Cat. No. IM56) (1 µg/ml). Detection: chemiluminescence.
References
ReferencesCottam, D. W. and Rees, R. C., 1993. Intl. J. Oncol. 2, 861.
Stetler-Stevenson, W. G., et al. 1993. FASEB J. 7, 1434.
Woessner, J. F., 1991. FASEB J. 5, 2145.
Liotta, L. A. and Stetler-Stevenson, W. G., 1990. in Sem. Cancer Biol., ed. M. M. Gottesman. Vol. 1(2), 99.
Product Information
DeclarationManufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.
FormLiquid
FormulationIn 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0
Negative controlTIMP-1 protein (Cat. No. PF019)
Positive controlhTIMP-2 protein (Cat. No. PF021)
Preservative≤0.1% sodium azide
Applications
Key Applications Frozen Sections
Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesFrozen Sections (1 µg/ml)
Immunoblotting (5 µg/ml)
Paraffin Sections (5 µg/ml, see application references)
Application CommentsTIMP-2 is found in most cells and tissues in low amounts. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide (YRGAAPPKQEFLDIED) corresponding to amino acids 178-193 in the C-terminal region of human TIMP-2
ImmunogenHuman
Clone67-4H11
HostMouse
IsotypeIgG₁
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
IM56 0

Documentation

Anti-TIMP-2 (Ab-2) Mouse mAb (67-4H11) Certificates of Analysis

TitleLot Number
IM56

References

Reference overview
Cottam, D. W. and Rees, R. C., 1993. Intl. J. Oncol. 2, 861.
Stetler-Stevenson, W. G., et al. 1993. FASEB J. 7, 1434.
Woessner, J. F., 1991. FASEB J. 5, 2145.
Liotta, L. A. and Stetler-Stevenson, W. G., 1990. in Sem. Cancer Biol., ed. M. M. Gottesman. Vol. 1(2), 99.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision05-September-2007 JSW
SynonymsAnti-Tissue Inhibitor of Metalloproteinase-2
ApplicationFrozen Sections (1 µg/ml)
Immunoblotting (5 µg/ml)
Paraffin Sections (5 µg/ml, see application references)
Application Data
Detection of hamster TIMP-2 by immunoblotting. Sample: Expressed in CHO cells. Primary antibody: Anti-TIMP-2 (Ab-2) Mouse mAb (67-4H11) (Cat. No. IM56) (1 µg/ml). Detection: chemiluminescence.
DescriptionPurified mouse monoclonal antibody (see application references). Recognizes the ~21-28 kDa TIMP-2 protein.
BackgroundMatrix metalloproteinases (MMPs) are a family of enzymes that are responsible for the degradation of extracellular matrix components such as collagen, laminin and proteoglycans. In addition to sequence homology, all MMPs share the following characteristics: the catalytic mechanism is dependent upon a zinc ion at the active center, they cleave one or more extracellular matrix components, they are secreted as zymogens which are activated by removal of an ~10 kDa segment from the N-terminus and they are inhibited by tissue inhibitor of metalloproteinases (TIMP). These enzymes are involved in normal physiological processes such as embryogenesis and tissue remodeling and may play an important role in arthritis, periodontitis, and metastasis. A family of endogenous inhibitors, tissue inhibitors of metalloproteinase (TIMP), regulate the activation and activity of MMPs. TIMPs are capable of altering the metastatic potential of cancer cells and have been shown to inhibit invasion and metastasis in animal models. TIMP-2 (also called CSC-21K) is a 21 kDa unglycosylated protein that is expressed by a variety of cell types. It forms a non-covalent, stoichiometric complex with both latent and active MMPs, showing a preference for MMP-2.
HostMouse
Immunogen speciesHuman
Immunogena synthetic peptide (YRGAAPPKQEFLDIED) corresponding to amino acids 178-193 in the C-terminal region of human TIMP-2
Clone67-4H11
IsotypeIgG₁
Speciesbovine, guinea pig, human, mouse, rabbit, rat
Positive controlhTIMP-2 protein (Cat. No. PF021)
Negative controlTIMP-1 protein (Cat. No. PF019)
FormLiquid
FormulationIn 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsTIMP-2 is found in most cells and tissues in low amounts. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesCottam, D. W. and Rees, R. C., 1993. Intl. J. Oncol. 2, 861.
Stetler-Stevenson, W. G., et al. 1993. FASEB J. 7, 1434.
Woessner, J. F., 1991. FASEB J. 5, 2145.
Liotta, L. A. and Stetler-Stevenson, W. G., 1990. in Sem. Cancer Biol., ed. M. M. Gottesman. Vol. 1(2), 99.