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DR1126 Anti-WARS Mouse mAb (3A12)

DR1126
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody
Description
OverviewRecognizes the ~50-52 kDa WARS protein in HeLa cells.
Catalogue NumberDR1126
Brand Family Calbiochem®
SynonymsAnti-Tryptophanyl-tRNA Synthetase
Application Data
Detection of human WARS by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-WARS Mouse mAb (3A12) (Cat. No. DR1126) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human WARS by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-WARS Mouse mAb (3A12) (Cat. No. DR1126) (10 µg/ml). Detection: fluorescence.
References
Product Information
Formliquid
FormulationIn PBS, pH 7.2.
Negative control293T cells
Positive controlHeLa cells
PreservativeNone
Applications
Key Applications Immunoblotting (Western Blotting)
Immunocytochemistry
Application NotesImmunoblotting (1-5 µg/ml) Immunocytochemsitry (10 µg/ml)
Application CommentsVariables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids 50-150 human WARS, expressed as a GST fusion protein
Clone3A12
HostMouse
IsotypeIgG2a
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
DR1126 0

Documentation

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision05-November-2010 RFH
SynonymsAnti-Tryptophanyl-tRNA Synthetase
ApplicationImmunoblotting (1-5 µg/ml) Immunocytochemsitry (10 µg/ml)
Application Data
Detection of human WARS by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-WARS Mouse mAb (3A12) (Cat. No. DR1126) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human WARS by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-WARS Mouse mAb (3A12) (Cat. No. DR1126) (10 µg/ml). Detection: fluorescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the ~50-52 kDa WARS protein.
BackgroundThis gene product catalyzes the aminoacylation of tRNA by their cognate amino acid. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression.
HostMouse
ImmunogenA recombinant polypeptide corresponding to amino acids 50-150 human WARS, expressed as a GST fusion protein
Clone3A12
IsotypeIgG2a
Specieshuman
Positive controlHeLa cells
Negative control293T cells
Formliquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsVariables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review