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ASK12 Bcl-2 Family Antibody Sampler Kit

ASK12
  
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      Overview

      Replacement Information

      Key Spec Table

      Description
      Overview

      This product has been discontinued.





      A collection of antibodies for studying the Bcl-2 family of proteins. Contains 20 µg of each of the following antibodies: Anti-Bcl-2 (Cat. Nos. OP60 and PC68), Anti-Bax (Cat. No. PC66), Anti-Bcl-x (Cat. Nos. PC67 and PC89), and Anti-Bak (Cat. Nos. AM03 and AM04).
      Please see individual product descriptions for more information.
      Catalogue NumberASK12
      Brand Family Calbiochem®
      Application Data
      References
      ReferencesChittenden, T., et al. 1995. Nature 374, 733.
      Craig, R.W., 1995. Sem. Cancer Biol. 6, 35.
      Hanada, M., et al. 1995. J. Biol. Chem. 270, 11962.
      Mentz, F., et al. 1994. Blood 84, 657.
      Oltvai, Z.N., et al. 1993. Cell 74, 609.
      Tsujimoto, Y., et al. 1985. Science 288, 1440.
      Product Information
      FormLiquid
      Applications
      Application NotesImmunoblotting (see characteristics below)
      Frozen Sections (see characteristics below)
      Paraffin Sections (see characteristics below)
      Application CommentsBcl-x (Ab-1) will react strongly with Bcl-xL and moderately with Bcl-xs; Bcl-xs (Ab-1) will not react to Bcl-xL. Brain sections can be used as a negative control for Bcl-2 and Bcl-xL (only random cells will stain positive); trp E (Ab-1) or normal rabbit IgG can also be used. MCF-7 cells treated with 0.2 mg/ml doxorubicin can be used as a positive control for Bcl-x (treat for 72 h) and Bax (treat for 48 h). The number and intensity of extra bands can be minimized by using 0.3% Tween®-20 detergent in the antibody and wash buffers. Antibodies should be titrated for optimal results in individual systems.
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Bcl-2 Family Antibody Sampler Kit Certificates of Analysis

      TitleLot Number
      ASK12

      References

      Reference overview
      Chittenden, T., et al. 1995. Nature 374, 733.
      Craig, R.W., 1995. Sem. Cancer Biol. 6, 35.
      Hanada, M., et al. 1995. J. Biol. Chem. 270, 11962.
      Mentz, F., et al. 1994. Blood 84, 657.
      Oltvai, Z.N., et al. 1993. Cell 74, 609.
      Tsujimoto, Y., et al. 1985. Science 288, 1440.

      Brochure

      Title
      Caspases and other Apoptosis Related Tools Brochure
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision26-February-2008 JSW
      ApplicationImmunoblotting (see characteristics below)
      Frozen Sections (see characteristics below)
      Paraffin Sections (see characteristics below)
      Application Data
      DescriptionSet of 7 antibodies specific for various members of the Bcl-2, Bax, Blc-x, Bcl-xs, and BAK.
      BackgroundThe Bcl-2 gene family is critical for regulation of apoptosis and contributes to the pathogenesis of many diseases. Constitutive expression of Bcl-2 in some lymphomas and leukemias causes escape of these cells from physiological apoptosis in the germinal centers and appears to be a major element of pathogenesis. Members of the Bcl-2 gene family can act as either effectors or inhibitors of apoptosis. Among the proteins which promote or induce apoptosis are such proteins as Bcl-xs, Bax, and Bak. The inhibitors of apoptosis include Bcl-2 itself and Bcl-xL. Members of the Bcl-2 family of proteins form homodimers as well as form heterodimers with other family members and it is the intracellular balance between these various dimers which appears to determine whether a cell enters into the apoptotic pathway.
      Specieshuman
      FormLiquid
      CommentsBcl-x (Ab-1) will react strongly with Bcl-xL and moderately with Bcl-xs; Bcl-xs (Ab-1) will not react to Bcl-xL. Brain sections can be used as a negative control for Bcl-2 and Bcl-xL (only random cells will stain positive); trp E (Ab-1) or normal rabbit IgG can also be used. MCF-7 cells treated with 0.2 mg/ml doxorubicin can be used as a positive control for Bcl-x (treat for 72 h) and Bax (treat for 48 h). The number and intensity of extra bands can be minimized by using 0.3% Tween®-20 detergent in the antibody and wash buffers. Antibodies should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesChittenden, T., et al. 1995. Nature 374, 733.
      Craig, R.W., 1995. Sem. Cancer Biol. 6, 35.
      Hanada, M., et al. 1995. J. Biol. Chem. 270, 11962.
      Mentz, F., et al. 1994. Blood 84, 657.
      Oltvai, Z.N., et al. 1993. Cell 74, 609.
      Tsujimoto, Y., et al. 1985. Science 288, 1440.