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71257 Insect RoboPop™ Ni-NTA His•Bind® Purification Kit

71257
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      The Insect RoboPop™ Ni-NTA His•Bind® Purification Kit is designed for filtration-based, 96-well format purification of His•Tag® fusion proteins directly from transfected insect cell cultures. This kit includes Insect PopCulture® Reagent for protein extraction from total cultures, Benzonase® Nuclease for viscosity reduction, Ni-NTA His•Bind Resin and buffers, 2-ml 96-well Filter Plate and Collection Plate with Sealer. The kit is configured for robotic processing of transfected 10-ml suspension cultures and purifies up to 400 µg His•Tag fusion protein per culture based on binding capacity of the resin. Protein yields ranging from 60 to 140 µg per 10 ml culture were obtained with β-gal, Fluc, MAP kinase, and cdc2 kinase expressed in the pIEx™ transient protein expression system (Loomis 2002).

      In practice, Insect PopCulture Reagent is added directly to the cell culture, followed by the addition of Benzonase Nuclease. The lysate is transferred to a tube of appropriate size or deep-well plate and Ni-NTA His•Bind resin is added. After mixing, the lysate-resin slurry is transferred to the Filter Plate, and washing and elution steps are carried out on a vacuum manifold. The Filter Plate is compatible with standard vacuum manifolds for either manual or automated processing, and the entire purification has been validated for robotic sample processing with the Packard-brand MultiPROBE® II liquid handling workstation from PerkinElmer Life Sciences and with the Genesis® Freedom™ workstation from Tecan. Also use the Insect RoboPop Ni-NTA His•Bind Purification Kit with centrifugation when a vacuum manifold is not available.




      Filter Plate
      The 2 ml 96-well Filter Plate that can be used with the filtration-based RoboPop Purification Kits is also available separately.

      Catalogue Number71257
      Brand Family Novagen®
      SynonymsInsectDirect™ System- Insect RoboPop™ Ni-NTA His•Bind® Purification Kit
      References
      ReferencesLoomis, K., et al. 2002. InNovations 15, 16.
      Product Information
      50 mlInsect PopCulture® Reagent
      10 KUBenzonase® Nuclease
      10 mlNi-NTA His•Bind Resin
      125 ml4X Ni-NTA Bind Buffer
      2 x 125 ml4X Ni-NTA Wash Buffer
      50 ml4X Ni-NTA Elute Buffer
      12-ml 96-well Filter Plate
      1Collection Plate with Sealer
      Note: 1 KU = 1000 units
      DeclarationRoboPop is a trademark and His•Bind is a registered trademark of EMD Biosciences, Inc., Madison, WI USA
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
      Storage Multiple storage requirements
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Insect RoboPop™ Ni-NTA His•Bind® Purification Kit Certificates of Analysis

      TitleLot Number
      71257

      References

      Reference overview
      Loomis, K., et al. 2002. InNovations 15, 16.

      Brochure

      Title
      Insect Directル Protein Expression & Purification System
      Protein Purification and Detection Tools

      Citations

      Title
    • Kathryn H. Loomis, et al. (2005) InsectDirectTM System: rapid, high-level protein expression and purication from insect cells. Journal of Structural and Functional Genomics 6, 189–194.
    • User Protocols

      Title
      TB368 Insect RoboPop™ Ni-NTA His•Bind® Purif. Kit