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482722 Nitric Oxide Synthase, Constitutive, Rat Neuronal, Recombinant, High Purity

482722
  
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      Overview

      Replacement Information

      Key Spec Table

      Description
      Overview

      This product has been discontinued.





      Recombinant, rat neuronal NOS expressed in S. frugiperda insect cells using a baculovirus expression system.
      Catalogue Number482722
      Brand Family Calbiochem®
      SynonymsnNOS, Rat, Neuronal Nitric Oxide Synthase
      References
      ReferencesAbu-Soud, H.M., et al. 1994. J. Biol. Chem. 269, 32047.
      Abu-Soud, H.M., et al. 1994. J. Biol. Chem. 269, 32318.
      Richards, M.K., and Marletta, M.A. 1994. Biochemistry 33, 14723.
      Sheta, E.A., et al. 1994. J. Biol. Chem. 269, 15147.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme that will produce 1 nmol of nitric oxide per min in the presence of calmodulin at 37°C, pH 7.5.
      FormLiquid
      FormulationIn 100 mM NaCl, 50 mM HEPES, 10 µM tetrahydro-L-biopterin, 20% glycerol, pH 7.4.
      Applications
      Biological Information
      Purity≥95% by SDS PAGE
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 20/21/22-36/37/38

      Harmful by inhalation, in contact with skin and if swallowed.
      Irritating to eyes, respiratory system and skin.
      S PhraseS: 26-36

      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Harmful
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Nitric Oxide Synthase, Constitutive, Rat Neuronal, Recombinant, High Purity Certificates of Analysis

      TitleLot Number
      482722

      References

      Reference overview
      Abu-Soud, H.M., et al. 1994. J. Biol. Chem. 269, 32047.
      Abu-Soud, H.M., et al. 1994. J. Biol. Chem. 269, 32318.
      Richards, M.K., and Marletta, M.A. 1994. Biochemistry 33, 14723.
      Sheta, E.A., et al. 1994. J. Biol. Chem. 269, 15147.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision12-August-2008 RFH
      SynonymsnNOS, Rat, Neuronal Nitric Oxide Synthase
      DescriptionRecombinant, rat neuronal NOS expressed in S. frugiperda insect cells using a baculovirus expression system. Useful for studies involving NOS regulation, inhibitor screening, and kinetic analysis.
      FormLiquid
      FormulationIn 100 mM NaCl, 50 mM HEPES, 10 µM tetrahydro-L-biopterin, 20% glycerol, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      Recommended reaction conditions
      Combine the following at 37°C: 50 mM        HEPES, pH 7.4 12 µM        BH4 1 mM         CaCl2 20 µg/ml   Calmodulin 100 µM     NADPH 50 µM      Arginine 120 µM     DTT 3 µM        Oxyhemoglobin Measure spectrophotometrically at 401 nm.
      Purity≥95% by SDS PAGE
      Unit definitionOne unit is defined as the amount of enzyme that will produce 1 nmol of nitric oxide per min in the presence of calmodulin at 37°C, pH 7.5.
      Storage ≤ -70°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Harmful
      ReferencesAbu-Soud, H.M., et al. 1994. J. Biol. Chem. 269, 32047.
      Abu-Soud, H.M., et al. 1994. J. Biol. Chem. 269, 32318.
      Richards, M.K., and Marletta, M.A. 1994. Biochemistry 33, 14723.
      Sheta, E.A., et al. 1994. J. Biol. Chem. 269, 15147.

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      Catalogue Number Description  
      482721 Nitric Oxide Synthase, Constitutive, Rat Neuronal, Recombinant, S. frugiperda Show Pricing & Availability

      Categories

      Life Science Research > Proteins and Enzymes > Other Enzymes