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PS1003 PhosphoDetect™ Anti-IFNAR1 (pSer535,539) Rabbit pAb

PS1003
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~130 kDa IFNAR1 protein phosphorylated at Ser535,539.
      Catalogue NumberPS1003
      Brand Family Calbiochem®
      Application Data
      Detection of human IFNAR1, phosphorylated at Ser535/539, by immunoblotting. Samples: Whole cell lysate from HEK293 cells mock transfected (lane 1), transfected with wild-type IFNAR1 (lane 2), or transfected with S535A/S539A mutant IFNAR1 (lane 3). Primary antibody: PhosphoDetect™ Anti-IFNAR1 (pSer535,539) Rabbit pAb (Cat. No. PS1003) (1:1000) pre-incubated without peptide (control, left panel), with immunogen phosphopeptide (phos-peptide, middle panel), or non-phosphopeptide corresponding to the phosphopeptide immunogen (dephos-peptide, right panel). Detection: chemiluminescence.
      References
      ReferencesSuresh Kumar, K.G., et al. 2003. EMBO J. 22, 5480.
      Kirkwood, J., et al. 2002. Semin. Oncol. 29, 18.
      Biron, C.A., et al. 2001. Immunity 14, 661.
      Stark, G.R., et al. 1998. Ann. Rev. Biochem. 67, 227.
      Uze, G., et al. 1990. Cell 60, 225.
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
      Positive controlIFNAR1 transfected HEK293 cells
      PreservativeNone
      Applications
      Key Applications Dot Blot
      Immunoblotting (Western Blotting)
      Application NotesDot Blot (1:1000)
      Immunoblotting (1:1000)
      Application CommentsDoes not recognize unphosphorylated IFNAR1. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser535,539 phosphorylation sites of human IFNAR1
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      PhosphoDetect™ Anti-IFNAR1 (pSer535,539) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PS1003

      References

      Reference overview
      Suresh Kumar, K.G., et al. 2003. EMBO J. 22, 5480.
      Kirkwood, J., et al. 2002. Semin. Oncol. 29, 18.
      Biron, C.A., et al. 2001. Immunity 14, 661.
      Stark, G.R., et al. 1998. Ann. Rev. Biochem. 67, 227.
      Uze, G., et al. 1990. Cell 60, 225.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision16-October-2007 RFH
      ApplicationDot Blot (1:1000)
      Immunoblotting (1:1000)
      Application Data
      Detection of human IFNAR1, phosphorylated at Ser535/539, by immunoblotting. Samples: Whole cell lysate from HEK293 cells mock transfected (lane 1), transfected with wild-type IFNAR1 (lane 2), or transfected with S535A/S539A mutant IFNAR1 (lane 3). Primary antibody: PhosphoDetect™ Anti-IFNAR1 (pSer535,539) Rabbit pAb (Cat. No. PS1003) (1:1000) pre-incubated without peptide (control, left panel), with immunogen phosphopeptide (phos-peptide, middle panel), or non-phosphopeptide corresponding to the phosphopeptide immunogen (dephos-peptide, right panel). Detection: chemiluminescence.
      DescriptionRabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen, followed by immunoaffinity chromatography. Recognizes the IFNAR1 protein phosphorylated at Ser535/539.
      BackgroundInterferons (IFNs) are important therapeutic agents. They have demonstrated activity in infectious autoimmune diseases and selected cancers. IFNs activate their target cells by first binding to specific receptors on the cell surface to induce synthesis of effector protein. There are three types of natural IFNs: alpha (α), beta (β), and gamma (γ). The first two are classified as type I because they bind to IFN cell surface receptor type I (IFNAR1). IFN γ belongs to type II interferons since it binds to type II receptors only. The stimulation of type I and type II IFNRs results in the activation of the Janus family protein tyrosine kinases (PTKs), i.e. Jak1 and Tyk2 PTKs for IFNAR1, and Jak1 and Jak2 PTKs for type II IFNR. Down-regulation of IFNAR1 plays a role in defining the magnitude and duration of cytokine signaling. This down-regulation is believed to be regulated, in part, by phosphorylation at Ser535 and Ser539.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser535,539 phosphorylation sites of human IFNAR1
      IsotypeIgG
      Speciesbovine, human, mouse, rat
      Positive controlIFNAR1 transfected HEK293 cells
      FormLiquid
      FormulationIn 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
      PreservativeNone
      CommentsDoes not recognize unphosphorylated IFNAR1. Antibody should be titrated for optimal results in individual systems.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesSuresh Kumar, K.G., et al. 2003. EMBO J. 22, 5480.
      Kirkwood, J., et al. 2002. Semin. Oncol. 29, 18.
      Biron, C.A., et al. 2001. Immunity 14, 661.
      Stark, G.R., et al. 1998. Ann. Rev. Biochem. 67, 227.
      Uze, G., et al. 1990. Cell 60, 225.