M6899
Murashige and Skoog Basal Salts with minimal organics
powder, suitable for plant cell culture
Synonym(s):
MSMO
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About This Item
NACRES:
NA.72
UNSPSC Code:
12352207
form
powder
Quality Level
technique(s)
cell culture | plant: suitable
application(s)
agriculture
storage temp.
2-8°C
Application
Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and the Kinetin (cytokinin/cell division promoter).
Preparation Note
Formulated to contain 4.4 grams of powder per liter of medium.
With the macro- and micronutrients, and vitamins as described by Linsmaier and Skoog (1965).
Media Formulation
Media Formulation
Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog Shoot Multiplication Medium A (M6899 is a variant media) with macro- and micronutrients, vitamins as described by Linsmaier and Skoog.
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Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Ox. Sol. 2
Storage Class
5.1B - Oxidizing hazardous materials
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type P3 (EN 143) respirator cartridges
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Li Wang et al.
Planta, 249(4), 1107-1118 (2018-12-16)
In hybrids of Arabidopsis, cotyledons influence the amount and proportion of hybrid vigor in total plant growth. We found Arabidopsis cotyledons are essential for plant growth and in some hybrids for hybrid vigor. In hybrids between C24 and Landsberg erecta
Thelma F Madzima et al.
Frontiers in plant science, 12, 694289-694289 (2021-09-08)
Plants respond to abiotic stress stimuli, such as water deprivation, through a hierarchical cascade that includes detection and signaling to mediate transcriptional and physiological changes. The phytohormone abscisic acid (ABA) is well-characterized for its regulatory role in these processes in
Alexandra Lešková et al.
PloS one, 15(8), e0237448-e0237448 (2020-08-14)
We established that Endosidin2 (ES2) affected the trafficking routes of both newly synthesized and endocytic pools of PIN-FORMED2 (PIN2) in Arabidopsis root epidermal cells. PIN2 populations accumulated in separated patches, which gradually merged into large and compact ES2 aggregates (ES2As).
Pyrroloquinoline quinone is a plant growth promotion factor produced by Pseudomonas fluorescens B16.
Okhee Choi et al.
Plant physiology, 146(2), 657-668 (2007-12-07)
Pseudomonas fluorescens B16 is a plant growth-promoting rhizobacterium. To determine the factors involved in plant growth promotion by this organism, we mutagenized wild-type strain B16 using OmegaKm elements and isolated one mutant, K818, which is defective in plant growth promotion
Organic growth factor requirements of tobacco tissue cultures.
Linsmaier, E.M. and Skoog, F.
Physiologia Plantarum, 18, 100-127 (1965)
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