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  • Neuromyelitis optica and anti-aquaporin-4 antibodies measured by an enzyme-linked immunosorbent assay.

Neuromyelitis optica and anti-aquaporin-4 antibodies measured by an enzyme-linked immunosorbent assay.

Journal of neuroimmunology (2008-05-09)
Sei Hayakawa, Masahiro Mori, Akiko Okuta, Akiko Kamegawa, Yoshinori Fujiyoshi, Yasumasa Yoshiyama, Kaoru Mitsuoka, Kenichi Ishibashi, Sei Sasaki, Takamichi Hattori, Satoshi Kuwabara
PMID18462810
ABSTRACT

NMO-IgG, a disease-specific autoantibody for neuromyelitis optica, recognizes aquaporin-4 (AQP4) and has been examined by indirect immunofluorescence assay. We developed an enzyme-linked immunosorbent assay (ELISA) to detect anti-AQP4 antibodies by establishing methods for expression in a baculovirus system and purification of recombinant AQP4 as antigen. Elevated anti-AQP4 antibody titers in serum were found in 15 (71%) of 21 patients with neuromyelitis optica, 4.3% of 46 patients with multiple sclerosis, none of 51 normal controls, and 2.6% of 115 patients with other neurological diseases. The ELISA system can be substituted for the conventional NMO-IgG assay.