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Merck

05-773

Anti-phospho-MYPT1 (Thr850) Antibody, clone SA19, rabbit monoclonal

clone SA19, Upstate®, from rabbit

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Product Name

Anti-phospho-MYPT1 (Thr850) Antibody, clone SA19, rabbit monoclonal, clone SA19, Upstate®, from rabbit

biological source

rabbit

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

SA19, monoclonal

species reactivity

human, chicken

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG

NCBI accession no.

NM_002480

UniProt accession no.

O14974

shipped in

wet ice

target post-translational modification

phosphorylation (pThr850)

Quality Level

100

Gene Information

human ... PPP1R12A(4659)

Analysis Note

Routinely evaluated by immunoblot on RIPA lysates from HEK293 cells phosphorylated by preincubation with ROKα/ROCK-II, active (Catalog # 14-338).

Application

Anti-phospho-MYPT1 (Thr850) Antibody, clone SA19 is an antibody against phospho-MYPT1 (Thr850) for use in WB.
Research Category
Signaling
Research Sub Category
Kinases & Phosphatases

Biochem/physiol Actions

MYPT1 phosphorylated on Thr850
Predicted to cross react with mouse and rat

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

doublet at 130kDa

Immunogen

KLH-conjugated, synthetic peptide containing the sequence ...RS[pT]GV..., in which [pT] corresponds to phosphorylated threonine at amino acid residue 850 of chicken myosin phosphatase target subunit 1 (MYPT1). Corresponds to Thr853 in human MYPT1.

Physical form

Format: Purified
Protein A purified

Preparation Note

2 years at -20°C from date of shipment

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

10 - Combustible liquids

wgk

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sustained therapeutic hypercapnia attenuates pulmonary arterial Rho-kinase activity and ameliorates chronic hypoxic pulmonary hypertension in juvenile rats.
Peng, G; Ivanovska, J; Kantores, C; Van Vliet, T; Engelberts, D; Kavanagh, BP; Enomoto et al.
American Journal of Physiology. Heart and Circulatory Physiology null
RhoA-mediated Ca2+ sensitization in erectile function.
Wang, Hua, et al.
The Journal of Biological Chemistry, 277, 30614-30621 (2002)
Role of protein phosphatase type 1 in contractile functions: myosin phosphatase.
Hartshorne, David J, et al.
The Journal of Biological Chemistry, 279, 37211-37214 (2004)
Hypertension and RhoA/Rho-kinase signaling in the vasculature: highlights from the recent literature.
Lee, Dexter L, et al.
Hypertension, 44, 796-799 (2004)
Myosin phosphatase: structure, regulation and function.
Ito, Masaaki, et al.
Molecular and Cellular Biochemistry, 259, 197-209 (2004)
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Global Trade Item Number

SKUGTIN
05-77304053252624681

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